1fzr: Difference between revisions

Revision as of 13:44, 21 February 2008

CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I

OverviewOverview

We have solved the crystal structure of the Holliday junction resolving enzyme T7 endonuclease I at 2.1 A resolution using the multiwavelength anomalous dispersion (MAD) technique. Endonuclease I exhibits strong structural specificity for four-way DNA junctions. The structure shows that it forms a symmetric homodimer arranged in two well-separated domains. Each domain, however, is composed of elements from both subunits, and amino acid side chains from both protomers contribute to the active site. While no significant structural similarity could be detected with any other junction resolving enzyme, the active site is similar to that found in several restriction endonucleases. T7 endonuclease I therefore represents the first crystal structure of a junction resolving enzyme that is a member of the nuclease superfamily of enzymes.

About this StructureAbout this Structure

1FZR is a Single protein structure of sequence from Bacteriophage t7. Active as Deoxyribonuclease IV (phage-T(4)-induced), with EC number 3.1.21.2 Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I., Hadden JM, Convery MA, Declais AC, Lilley DM, Phillips SE, Nat Struct Biol. 2001 Jan;8(1):62-7. PMID:11135673

Page seeded by OCA on Thu Feb 21 12:44:23 2008

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OCA

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-[[Image:1fzr.gif|left|200px]]<br /><applet load="1fzr" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1fzr.gif|left|200px]]<br /><applet load="1fzr" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1fzr, resolution 2.10&Aring;" />
 '''CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I'''<br />
 ==Overview==
-We have solved the crystal structure of the Holliday junction resolving, enzyme T7 endonuclease I at 2.1 A resolution using the multiwavelength, anomalous dispersion (MAD) technique. Endonuclease I exhibits strong, structural specificity for four-way DNA junctions. The structure shows, that it forms a symmetric homodimer arranged in two well-separated, domains. Each domain, however, is composed of elements from both subunits, and amino acid side chains from both protomers contribute to the active, site. While no significant structural similarity could be detected with, any other junction resolving enzyme, the active site is similar to that, found in several restriction endonucleases. T7 endonuclease I therefore, represents the first crystal structure of a junction resolving enzyme that, is a member of the nuclease superfamily of enzymes.
+We have solved the crystal structure of the Holliday junction resolving enzyme T7 endonuclease I at 2.1 A resolution using the multiwavelength anomalous dispersion (MAD) technique. Endonuclease I exhibits strong structural specificity for four-way DNA junctions. The structure shows that it forms a symmetric homodimer arranged in two well-separated domains. Each domain, however, is composed of elements from both subunits, and amino acid side chains from both protomers contribute to the active site. While no significant structural similarity could be detected with any other junction resolving enzyme, the active site is similar to that found in several restriction endonucleases. T7 endonuclease I therefore represents the first crystal structure of a junction resolving enzyme that is a member of the nuclease superfamily of enzymes.
 ==About this Structure==
-FZR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_IV_(phage-T(4)-induced) Deoxyribonuclease IV (phage-T(4)-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.2 3.1.21.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FZR OCA].
+FZR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_IV_(phage-T(4)-induced) Deoxyribonuclease IV (phage-T(4)-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.2 3.1.21.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FZR OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Deoxyribonuclease IV (phage-T(4)-induced)]]
 [[Category: Single protein]]
-[[Category: Convery, M.A.]]
+[[Category: Convery, M A.]]
-[[Category: Declais, A.C.]]
+[[Category: Declais, A C.]]
-[[Category: Hadden, J.M.]]
+[[Category: Hadden, J M.]]
-[[Category: Lilley, D.M.J.]]
+[[Category: Lilley, D M.J.]]
-[[Category: Phillips, S.E.V.]]
+[[Category: Phillips, S E.V.]]
 [[Category: composite active site]]
 [[Category: domain swapped]]
@@ Line 24: / Line 24: @@
 [[Category: homodimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:33:31 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:23 2008''