4gsp: Difference between revisions
New page: left|200px<br /> <applet load="4gsp" size="450" color="white" frame="true" align="right" spinBox="true" caption="4gsp, resolution 1.65Å" /> '''RIBONUCLEASE T1 COM... |
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==About this Structure== | ==About this Structure== | ||
4GSP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]] with CA and 3GP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4GSP OCA]]. | 4GSP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]] with CA and 3GP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]]. Structure known Active Sites: BI1, CAL and CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4GSP OCA]]. | ||
==Reference== | ==Reference== | ||
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9546218 9546218] | Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9546218 9546218] | ||
[[Category: Aspergillus oryzae]] | [[Category: Aspergillus oryzae]] | ||
[[Category: Ribonuclease T(1)]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
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Revision as of 13:46, 30 October 2007
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RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS
OverviewOverview
Here we present a time-resolved crystallographic analysis of the, hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1., The use of a slow substrate and fast crystallization methods made it, possible to perform the study with conventional data-collection, techniques. The results support the idea that the hydrolysis reaction, proceeds through a mechanism that is the inverse of the, transesterification reaction. In addition, the structures provide an, explanation for the differential behavior of RNase T1 towards exo- and, endo-cyclic thiophosphates.
About this StructureAbout this Structure
4GSP is a [Single protein] structure of sequence from [Aspergillus oryzae] with CA and 3GP as [ligands]. Active as [Ribonuclease T(1)], with EC number [3.1.27.3]. Structure known Active Sites: BI1, CAL and CAT. Full crystallographic information is available from [OCA].
ReferenceReference
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218
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