1fi8: Difference between revisions
New page: left|200px<br /><applet load="1fi8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fi8, resolution 2.2Å" /> '''RAT GRANZYME B [N66Q]... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1fi8.gif|left|200px]]<br /><applet load="1fi8" size=" | [[Image:1fi8.gif|left|200px]]<br /><applet load="1fi8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1fi8, resolution 2.2Å" /> | caption="1fi8, resolution 2.2Å" /> | ||
'''RAT GRANZYME B [N66Q] COMPLEXED TO ECOTIN [81-84 IEPD]'''<br /> | '''RAT GRANZYME B [N66Q] COMPLEXED TO ECOTIN [81-84 IEPD]'''<br /> | ||
==Overview== | ==Overview== | ||
Granzyme B is a serine protease of the chymotrypsin fold that mediates | Granzyme B is a serine protease of the chymotrypsin fold that mediates cell death by cytotoxic lymphocytes. It is a processing enzyme, requiring extended peptide substrates containing an Asp residue. The determinants that allow for this substrate specificity are revealed in the three-dimensional structure of granzyme B in complex with a macromolecular inhibitor. The primary specificity for Asp occurs through a side-on interaction with Arg 226, a buried Arg side chain of granzyme B. An additional nine amino acids make contact with the substrate and define the granzyme B extended substrate specificity profile. The substrate determinants found in this structure are shared by other members of this protein class and help to reveal the properties that define substrate specificity. | ||
==About this Structure== | ==About this Structure== | ||
1FI8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | 1FI8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FI8 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Craik, C | [[Category: Craik, C S.]] | ||
[[Category: Fletterick, R | [[Category: Fletterick, R J.]] | ||
[[Category: Harris, J | [[Category: Harris, J L.]] | ||
[[Category: Waugh, S | [[Category: Waugh, S M.]] | ||
[[Category: beta strand structure]] | [[Category: beta strand structure]] | ||
[[Category: chymotrypsin fold]] | [[Category: chymotrypsin fold]] | ||
| Line 25: | Line 25: | ||
[[Category: protease substrate interactions]] | [[Category: protease substrate interactions]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:38:57 2008'' | ||
Revision as of 13:38, 21 February 2008
|
RAT GRANZYME B [N66Q] COMPLEXED TO ECOTIN [81-84 IEPD]
OverviewOverview
Granzyme B is a serine protease of the chymotrypsin fold that mediates cell death by cytotoxic lymphocytes. It is a processing enzyme, requiring extended peptide substrates containing an Asp residue. The determinants that allow for this substrate specificity are revealed in the three-dimensional structure of granzyme B in complex with a macromolecular inhibitor. The primary specificity for Asp occurs through a side-on interaction with Arg 226, a buried Arg side chain of granzyme B. An additional nine amino acids make contact with the substrate and define the granzyme B extended substrate specificity profile. The substrate determinants found in this structure are shared by other members of this protein class and help to reveal the properties that define substrate specificity.
About this StructureAbout this Structure
1FI8 is a Protein complex structure of sequences from Escherichia coli and Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
The structure of the pro-apoptotic protease granzyme B reveals the molecular determinants of its specificity., Waugh SM, Harris JL, Fletterick R, Craik CS, Nat Struct Biol. 2000 Sep;7(9):762-5. PMID:10966646
Page seeded by OCA on Thu Feb 21 12:38:57 2008