1f97: Difference between revisions

Revision as of 13:36, 21 February 2008

SOLUBLE PART OF THE JUNCTION ADHESION MOLECULE FROM MOUSE

OverviewOverview

Junctional adhesion molecules (JAMs) are a family of immunoglobulin-like single-span transmembrane molecules that are expressed in endothelial cells, epithelial cells, leukocytes and myocardia. JAM has been suggested to contribute to the adhesive function of tight junctions and to regulate leukocyte trans migration. We describe the crystal structure of the recombinant extracellular part of mouse JAM (rsJAM) at 2.5 A resolution. rsJAM consists of two immunoglobulin-like domains that are connected by a conformationally restrained short linker. Two rsJAM molecules form a U-shaped dimer with highly complementary interactions between the N-terminal domains. Two salt bridges are formed in a complementary manner by a novel dimerization motif, R(V,I,L)E, which is essential for the formation of rsJAM dimers in solution and common to the known members of the JAM family. Based on the crystal packing and studies with mutant rsJAM, we propose a model for homophilic adhesion of JAM. In this model, U-shaped JAM dimers are oriented in cis on the cell surface and form a two-dimensional network by trans-interactions of their N-terminal domains with JAM dimers from an opposite cell surface.

About this StructureAbout this Structure

1F97 is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

X-ray structure of junctional adhesion molecule: structural basis for homophilic adhesion via a novel dimerization motif., Kostrewa D, Brockhaus M, D'Arcy A, Dale GE, Nelboeck P, Schmid G, Mueller F, Bazzoni G, Dejana E, Bartfai T, Winkler FK, Hennig M, EMBO J. 2001 Aug 15;20(16):4391-8. PMID:11500366

Page seeded by OCA on Thu Feb 21 12:36:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1f97.gif|left|200px]]<br /><applet load="1f97" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1f97.gif|left|200px]]<br /><applet load="1f97" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1f97, resolution 2.5&Aring;" />
 '''SOLUBLE PART OF THE JUNCTION ADHESION MOLECULE FROM MOUSE'''<br />
 ==Overview==
-Junctional adhesion molecules (JAMs) are a family of immunoglobulin-like, single-span transmembrane molecules that are expressed in endothelial, cells, epithelial cells, leukocytes and myocardia. JAM has been suggested, to contribute to the adhesive function of tight junctions and to regulate, leukocyte trans migration. We describe the crystal structure of the, recombinant extracellular part of mouse JAM (rsJAM) at 2.5 A resolution., rsJAM consists of two immunoglobulin-like domains that are connected by a, conformationally restrained short linker. Two rsJAM molecules form a, U-shaped dimer with highly complementary interactions between the, N-terminal domains. Two salt bridges are formed in a complementary manner, by a novel dimerization motif, R(V,I,L)E, which is essential for the, formation of rsJAM dimers in solution and common to the known members of, the JAM family. Based on the crystal packing and studies with mutant, rsJAM, we propose a model for homophilic adhesion of JAM. In this model, U-shaped JAM dimers are oriented in cis on the cell surface and form a, two-dimensional network by trans-interactions of their N-terminal domains, with JAM dimers from an opposite cell surface.
+Junctional adhesion molecules (JAMs) are a family of immunoglobulin-like single-span transmembrane molecules that are expressed in endothelial cells, epithelial cells, leukocytes and myocardia. JAM has been suggested to contribute to the adhesive function of tight junctions and to regulate leukocyte trans migration. We describe the crystal structure of the recombinant extracellular part of mouse JAM (rsJAM) at 2.5 A resolution. rsJAM consists of two immunoglobulin-like domains that are connected by a conformationally restrained short linker. Two rsJAM molecules form a U-shaped dimer with highly complementary interactions between the N-terminal domains. Two salt bridges are formed in a complementary manner by a novel dimerization motif, R(V,I,L)E, which is essential for the formation of rsJAM dimers in solution and common to the known members of the JAM family. Based on the crystal packing and studies with mutant rsJAM, we propose a model for homophilic adhesion of JAM. In this model, U-shaped JAM dimers are oriented in cis on the cell surface and form a two-dimensional network by trans-interactions of their N-terminal domains with JAM dimers from an opposite cell surface.
 ==About this Structure==
-F97 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F97 OCA].
+F97 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F97 OCA].
 ==Reference==
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 [[Category: Mus musculus]]
 [[Category: Single protein]]
-[[Category: Arcy, A.D.]]
+[[Category: Arcy, A D.]]
 [[Category: Bazzoni, G.]]
 [[Category: Brockhaus, M.]]
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 [[Category: immunoglobulin superfamily]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:43:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:36:12 2008''