1ebo: Difference between revisions

Revision as of 13:26, 21 February 2008

CRYSTAL STRUCTURE OF THE EBOLA VIRUS MEMBRANE-FUSION SUBUNIT, GP2, FROM THE ENVELOPE GLYCOPROTEIN ECTODOMAIN

OverviewOverview

We have determined the structure of GP2 from the Ebola virus membrane fusion glycoprotein by X-ray crystallography. The molecule contains a central triple-stranded coiled coil followed by a disulfide-bonded loop homologous to an immunosuppressive sequence in retroviral glycoproteins, which reverses the chain direction and connects to an alpha helix packed antiparallel to the core helices. The structure suggests that fusion peptides near the N termini form disulfide-bonded loops at one end of the molecule and that the C-terminal membrane anchors are at the same end. In this conformation, GP2 could both bridge two membranes and facilitate their apposition to initiate membrane fusion. We also find a heptad irregularity like that in low-pH-induced influenza HA2 and a solvent ion trapped in a coiled coil like that in retroviral TMs.

About this StructureAbout this Structure

1EBO is a Single protein structure of sequence from Ebola virus sp. with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the Ebola virus membrane fusion subunit, GP2, from the envelope glycoprotein ectodomain., Weissenhorn W, Carfi A, Lee KH, Skehel JJ, Wiley DC, Mol Cell. 1998 Nov;2(5):605-16. PMID:9844633

Page seeded by OCA on Thu Feb 21 12:26:01 2008

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OCA

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-[[Image:1ebo.gif|left|200px]]<br /><applet load="1ebo" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1ebo, resolution 3.0&Aring;" />
 '''CRYSTAL STRUCTURE OF THE EBOLA VIRUS MEMBRANE-FUSION SUBUNIT, GP2, FROM THE ENVELOPE GLYCOPROTEIN ECTODOMAIN'''<br />
 ==Overview==
-We have determined the structure of GP2 from the Ebola virus membrane, fusion glycoprotein by X-ray crystallography. The molecule contains a, central triple-stranded coiled coil followed by a disulfide-bonded loop, homologous to an immunosuppressive sequence in retroviral glycoproteins, which reverses the chain direction and connects to an alpha helix packed, antiparallel to the core helices. The structure suggests that fusion, peptides near the N termini form disulfide-bonded loops at one end of the, molecule and that the C-terminal membrane anchors are at the same end. In, this conformation, GP2 could both bridge two membranes and facilitate, their apposition to initiate membrane fusion. We also find a heptad, irregularity like that in low-pH-induced influenza HA2 and a solvent ion, trapped in a coiled coil like that in retroviral TMs.
+We have determined the structure of GP2 from the Ebola virus membrane fusion glycoprotein by X-ray crystallography. The molecule contains a central triple-stranded coiled coil followed by a disulfide-bonded loop homologous to an immunosuppressive sequence in retroviral glycoproteins, which reverses the chain direction and connects to an alpha helix packed antiparallel to the core helices. The structure suggests that fusion peptides near the N termini form disulfide-bonded loops at one end of the molecule and that the C-terminal membrane anchors are at the same end. In this conformation, GP2 could both bridge two membranes and facilitate their apposition to initiate membrane fusion. We also find a heptad irregularity like that in low-pH-induced influenza HA2 and a solvent ion trapped in a coiled coil like that in retroviral TMs.
 ==About this Structure==
-EBO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ebola_virus_sp. Ebola virus sp.] with ZN and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EBO OCA].
+EBO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ebola_virus_sp. Ebola virus sp.] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBO OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Carfi, A.]]
-[[Category: Lee, K.H.]]
+[[Category: Lee, K H.]]
-[[Category: Skehel, J.J.]]
+[[Category: Skehel, J J.]]
 [[Category: Weissenhorn, W.]]
-[[Category: Wiley, D.C.]]
+[[Category: Wiley, D C.]]
 [[Category: CL]]
 [[Category: ZN]]
 [[Category: membrane fusion subunit]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:52:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:01 2008''