1dk1: Difference between revisions

Revision as of 13:17, 21 February 2008

DETAILED VIEW OF A KEY ELEMENT OF THE RIBOSOME ASSEMBLY: CRYSTAL STRUCTURE OF THE S15-RRNA COMPLEX

OverviewOverview

In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. Here we report the 2.8 inverted question mark resolution crystal structure of the highly conserved S15-rRNA complex. Protein S15 interacts in the minor groove with a G-U/G-C motif and a three-way junction. The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. The present structure gives insights into the dual role of S15 in ribosome assembly and translational regulation.

About this StructureAbout this Structure

1DK1 is a Single protein structure of sequence from Thermus thermophilus with , and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the S15-rRNA complex., Nikulin A, Serganov A, Ennifar E, Tishchenko S, Nevskaya N, Shepard W, Portier C, Garber M, Ehresmann B, Ehresmann C, Nikonov S, Dumas P, Nat Struct Biol. 2000 Apr;7(4):273-7. PMID:10742169

Page seeded by OCA on Thu Feb 21 12:17:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1dk1.gif|left|200px]]<br /><applet load="1dk1" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1dk1.gif|left|200px]]<br /><applet load="1dk1" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1dk1, resolution 2.80&Aring;" />
 '''DETAILED VIEW OF A KEY ELEMENT OF THE RIBOSOME ASSEMBLY: CRYSTAL STRUCTURE OF THE S15-RRNA COMPLEX'''<br />
 ==Overview==
-In bacterial ribosomes, the small (30S) ribosomal subunit is composed of, 16S rRNA and 21 distinct proteins. Ribosomal protein S15 is of particular, interest because it binds primarily to 16S rRNA and is required for, assembly of the small subunit and for intersubunit association, thus, representing a key element in the assembly of a whole ribosome. Here we, report the 2.8 inverted question mark resolution crystal structure of the, highly conserved S15-rRNA complex. Protein S15 interacts in the minor, groove with a G-U/G-C motif and a three-way junction. The latter is, constrained by a conserved base triple and stacking interactions, and, locked into place by magnesium ions and protein side chains, mainly, through interactions with the unique three-dimensional geometry of the, backbone. The present structure gives insights into the dual role of S15, in ribosome assembly and translational regulation.
+In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. Here we report the 2.8 inverted question mark resolution crystal structure of the highly conserved S15-rRNA complex. Protein S15 interacts in the minor groove with a G-U/G-C motif and a three-way junction. The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. The present structure gives insights into the dual role of S15 in ribosome assembly and translational regulation.
 ==About this Structure==
-DK1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MG, NA and K as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DK1 OCA].
+DK1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DK1 OCA].
 ==Reference==
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 [[Category: x-ray]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:20:51 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:21 2008''