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| ==Overview==
| | {{ABSTRACT_17128971}} |
| We describe X-ray crystal and NMR solution structures of the protein coded for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal structure was determined to 1.15 A with an R factor of 14.9% (Rfree = 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide derivatized crystals. The ensemble of NMR conformers was determined with protein samples labeled with 15N and 13C + 15N. The X-ray structure and NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93. At1g77540 was found to adopt a fold similar to that of GCN5-related N-acetyltransferases. Enzymatic activity assays established that At1g77540 possesses weak acetyltransferase activity against histones H3 and H4. Chemical shift perturbations observed in 15N-HSQC spectra upon the addition of CoA indicated that the cofactor binds and identified its binding site. The molecular details of this interaction were further elucidated by solving the X-ray structure of the At1g77540-CoA complex. This work establishes that the domain family COG2388 represents a novel class of acetyltransferase and provides insight into possible mechanistic roles of the conserved Cys76 and His41 residues of this family.
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| ==About this Structure== | | ==About this Structure== |
| 1XMT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMT OCA]. | | 1XMT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMT OCA]. |
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| ==Reference==
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| Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17128971 17128971]
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| [[Category: Arabidopsis thaliana]] | | [[Category: Arabidopsis thaliana]] |
| [[Category: Single protein]] | | [[Category: Single protein]] |
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| [[Category: Putative acetyltransferase]] | | [[Category: Putative acetyltransferase]] |
| [[Category: Structural genomic]] | | [[Category: Structural genomic]] |
| | [[Category: Unknown function]] |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 15:04:49 2008'' | | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 18:37:45 2008'' |