2bs9: Difference between revisions
New page: left|200px<br /> <applet load="2bs9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bs9, resolution 2.20Å" /> '''NATIVE CRYSTAL STRU... |
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==About this Structure== | ==About this Structure== | ||
2BS9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | 2BS9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Xylan_1,4-beta-xylosidase Xylan 1,4-beta-xylosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.37 3.2.1.37]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BS9 OCA]]. | ||
==Reference== | ==Reference== | ||
Enzyme-substrate complex structures of a GH39 beta-xylosidase from Geobacillus stearothermophilus., Czjzek M, Ben David A, Bravman T, Shoham G, Henrissat B, Shoham Y, J Mol Biol. 2005 Nov 4;353(4):838-46. Epub 2005 Sep 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16212978 16212978] | Enzyme-substrate complex structures of a GH39 beta-xylosidase from Geobacillus stearothermophilus., Czjzek M, Ben David A, Bravman T, Shoham G, Henrissat B, Shoham Y, J Mol Biol. 2005 Nov 4;353(4):838-46. Epub 2005 Sep 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16212978 16212978] | ||
[[Category: | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Xylan 1,4-beta-xylosidase]] | |||
[[Category: Bravman, T.]] | [[Category: Bravman, T.]] | ||
[[Category: Czjzek, M.]] | [[Category: Czjzek, M.]] | ||
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[[Category: thermophilic enzyme]] | [[Category: thermophilic enzyme]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:40:59 2007'' | ||
Revision as of 13:36, 30 October 2007
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NATIVE CRYSTAL STRUCTURE OF A GH39 BETA-XYLOSIDASE XYNB1 FROM GEOBACILLUS STEAROTHERMOPHILUS
OverviewOverview
Beta-D-Xylosidases are glycoside hydrolases that catalyse the release of, xylose units from short xylooligosaccharides and are engaged in the final, breakdown of plant cell-wall hemicelluloses. beta-D-Xylosidases are found, in glycoside hydrolase families 3, 39, 43, 52 and 54. The first crystal, structure of a GH39 beta-xylosidase revealed a multi-domain organization, with the catalytic domain having the canonical (beta/alpha)8 barrel fold., Here, we report the crystal structure of the GH39 Geobacillus, stearothermophilus beta-D-xylosidase, inactivated by a point mutation of, the general acid-base residue E160A, in complex with the chromogenic, substrate molecule 2,5-dinitrophenyl-beta-D-xyloside. Surprisingly, six of, the eight active sites present in the crystallographic asymmetric ... [(full description)]
About this StructureAbout this Structure
2BS9 is a [Single protein] structure of sequence from [Geobacillus stearothermophilus] with CA as [ligand]. Active as [Xylan 1,4-beta-xylosidase], with EC number [3.2.1.37]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Enzyme-substrate complex structures of a GH39 beta-xylosidase from Geobacillus stearothermophilus., Czjzek M, Ben David A, Bravman T, Shoham G, Henrissat B, Shoham Y, J Mol Biol. 2005 Nov 4;353(4):838-46. Epub 2005 Sep 20. PMID:16212978
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