1cy8: Difference between revisions

Revision as of 13:11, 21 February 2008

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE AND 3'-THYMIDINE MONOPHOSPHATE

OverviewOverview

DNA topoisomerases are the enzymes responsible for controlling and maintaining the topological states of DNA. Type IA enzymes work by transiently breaking the phosphodiester backbone of one strand to allow passage of another strand through the break. The protein has to perform complex rearrangements of the DNA, and hence it is likely that different regions of the enzyme bind DNA with different affinities. In order to identify some of the DNA binding sites in the protein, we have solved the structures of several complexes of the 67 kDa N-terminal fragment of Escherichia coli DNA topoisomerase I with mono- and trinucleotides. There are five different binding sites in the complexes, one of which is adjacent to the active site. Two other sites are in the central hole of the protein and may represent general DNA binding regions. The positions of these sites allow us to identify different DNA binding regions and to understand their possible roles in the catalytic cycle.

About this StructureAbout this Structure

1CY8 is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as DNA topoisomerase, with EC number 5.99.1.2 Full crystallographic information is available from OCA.

ReferenceReference

Protein-nucleotide interactions in E. coli DNA topoisomerase I., Feinberg H, Changela A, Mondragon A, Nat Struct Biol. 1999 Oct;6(10):961-8. PMID:10504732

Page seeded by OCA on Thu Feb 21 12:10:58 2008

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OCA

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-[[Image:1cy8.gif|left|200px]]<br /><applet load="1cy8" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1cy8.gif|left|200px]]<br /><applet load="1cy8" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1cy8, resolution 2.45&Aring;" />
 '''COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE AND 3'-THYMIDINE MONOPHOSPHATE'''<br />
 ==Overview==
-DNA topoisomerases are the enzymes responsible for controlling and, maintaining the topological states of DNA. Type IA enzymes work by, transiently breaking the phosphodiester backbone of one strand to allow, passage of another strand through the break. The protein has to perform, complex rearrangements of the DNA, and hence it is likely that different, regions of the enzyme bind DNA with different affinities. In order to, identify some of the DNA binding sites in the protein, we have solved the, structures of several complexes of the 67 kDa N-terminal fragment of, Escherichia coli DNA topoisomerase I with mono- and trinucleotides. There, are five different binding sites in the complexes, one of which is, adjacent to the active site. Two other sites are in the central hole of, the protein and may represent general DNA binding regions. The positions, of these sites allow us to identify different DNA binding regions and to, understand their possible roles in the catalytic cycle.
+DNA topoisomerases are the enzymes responsible for controlling and maintaining the topological states of DNA. Type IA enzymes work by transiently breaking the phosphodiester backbone of one strand to allow passage of another strand through the break. The protein has to perform complex rearrangements of the DNA, and hence it is likely that different regions of the enzyme bind DNA with different affinities. In order to identify some of the DNA binding sites in the protein, we have solved the structures of several complexes of the 67 kDa N-terminal fragment of Escherichia coli DNA topoisomerase I with mono- and trinucleotides. There are five different binding sites in the complexes, one of which is adjacent to the active site. Two other sites are in the central hole of the protein and may represent general DNA binding regions. The positions of these sites allow us to identify different DNA binding regions and to understand their possible roles in the catalytic cycle.
 ==About this Structure==
-CY8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4, T3P and TMP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CY8 OCA].
+CY8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=T3P:'>T3P</scene> and <scene name='pdbligand=TMP:'>TMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CY8 OCA].
 ==Reference==
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 [[Category: relaxing enzyme]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:50:45 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:58 2008''