1e6a: Difference between revisions
New page: left|200px<br /> <applet load="1e6a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e6a, resolution 1.90Å" /> '''FLUORIDE-INHIBITED ... |
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==About this Structure== | ==About this Structure== | ||
1E6A is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with MN, F, NA, PO4, POP and POP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E6A OCA]]. | 1E6A is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with MN, F, NA, PO4, POP and POP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1]]. Structure known Active Sites: POA and POB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E6A OCA]]. | ||
==Reference== | ==Reference== | ||
Toward a quantum-mechanical description of metal-assisted phosphoryl transfer in pyrophosphatase., Heikinheimo P, Tuominen V, Ahonen AK, Teplyakov A, Cooperman BS, Baykov AA, Lahti R, Goldman A, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3121-6. Epub 2001 Mar 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11248042 11248042] | Toward a quantum-mechanical description of metal-assisted phosphoryl transfer in pyrophosphatase., Heikinheimo P, Tuominen V, Ahonen AK, Teplyakov A, Cooperman BS, Baykov AA, Lahti R, Goldman A, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3121-6. Epub 2001 Mar 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11248042 11248042] | ||
[[Category: Inorganic diphosphatase]] | |||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: phosphoryl transfer]] | [[Category: phosphoryl transfer]] | ||
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Revision as of 13:34, 30 October 2007
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FLUORIDE-INHIBITED SUBSTRATE COMPLEX OF SACCHAROMYCES CEREVISIAE INORGANIC PYROPHOSPHATASE
OverviewOverview
The wealth of kinetic and structural information makes inorganic, pyrophosphatases (PPases) a good model system to study the details of, enzymatic phosphoryl transfer. The enzyme accelerates metal-complexed, phosphoryl transfer 10(10)-fold: but how? Our structures of the yeast, PPase product complex at 1.15 A and fluoride-inhibited complex at 1.9 A, visualize the active site in three different states: substrate-bound, immediate product bound, and relaxed product bound. These span the steps, around chemical catalysis and provide strong evidence that a water, molecule (O(nu)) directly attacks PPi with a pK(a) vastly lowered by, coordination to two metal ions and D117. They also suggest that a, low-barrier hydrogen bond (LBHB) forms between D117 and O(nu), in part, because of steric crowding ... [(full description)]
About this StructureAbout this Structure
1E6A is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with MN, F, NA, PO4, POP and POP as [ligands]. Active as [Inorganic diphosphatase], with EC number [3.6.1.1]. Structure known Active Sites: POA and POB. Full crystallographic information is available from [OCA].
ReferenceReference
Toward a quantum-mechanical description of metal-assisted phosphoryl transfer in pyrophosphatase., Heikinheimo P, Tuominen V, Ahonen AK, Teplyakov A, Cooperman BS, Baykov AA, Lahti R, Goldman A, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3121-6. Epub 2001 Mar 6. PMID:11248042
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