2q9g: Difference between revisions

Revision as of 10:00, 6 August 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2q9g.png

Template:STRUCTURE 2q9g

Crystal structure of human cytochrome P450 46A1Crystal structure of human cytochrome P450 46A1

Template:ABSTRACT PUBMED 18621681

About this StructureAbout this Structure

2Q9G is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of substrate-bound and substrate-free cytochrome P450 46A1, the principal cholesterol hydroxylase in the brain., Mast N, White MA, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9546-51. Epub 2008 Jul 9. PMID:18621681

Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1., White MA, Mast N, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):487-95. Epub 2008, Apr 19. PMID:18453684

Page seeded by OCA on Wed Aug 6 10:00:31 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 {{STRUCTURE_2q9g|  PDB=2q9g  |  SCENE=  }}
-'''Crystal structure of human cytochrome P450 46A1'''
+===Crystal structure of human cytochrome P450 46A1===
-==Overview==
+<!--
-Human cytochrome P450 46A1 (CYP46A1) is one of the key enzymes in cholesterol homeostasis in the brain. The crystallization and heavy-atom structure solution of an active truncated CYP46A1 in complex with the high-affinity substrate analogue cholesterol-3-sulfate (CH-3S) is reported. The 2.6 angstroms structure of CYP46A1-CH-3S was solved using both anion and cation heavy-atom salts. In addition to the native anomalous signal from the haem iron, an NaI anion halide salt derivative and a complementary CsCl alkali-metal cation salt derivative were used. The general implications of the use of halide and alkali-metal quick soaks are discussed. The importance of using isoionic strength buffers, the titration of heavy-atom salts into different ionic species and the role of concentration are considered. It was observed that cation/anion-binding sites will occasionally overlap, which could negatively impact upon mixed RbBr soaks used for multiple anomalous scatterer MAD (MMAD). The use of complementary cation and anion heavy-atom salt derivatives is a convenient and powerful tool for MIR(AS) structure solution.
+The line below this paragraph, {{ABSTRACT_PUBMED_18621681}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18621681 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18621681}}
 ==About this Structure==
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 ==Reference==
+Crystal structures of substrate-bound and substrate-free cytochrome P450 46A1, the principal cholesterol hydroxylase in the brain., Mast N, White MA, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9546-51. Epub 2008 Jul 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18621681 18621681]
 Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1., White MA, Mast N, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):487-95. Epub 2008, Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18453684 18453684]
 [[Category: Cholesterol 24-hydroxylase]]
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 [[Category: P450]]
 [[Category: P450 46a1]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:03:17 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug  6 10:00:31 2008''