3d24: Difference between revisions

Revision as of 20:29, 27 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:3d24.png

Template:STRUCTURE 3d24

Crystal structure of ligand-binding domain of estrogen-related receptor alpha (ERRalpha) in complex with the peroxisome proliferators-activated receptor coactivator-1alpha box3 peptide (PGC-1alpha)Crystal structure of ligand-binding domain of estrogen-related receptor alpha (ERRalpha) in complex with the peroxisome proliferators-activated receptor coactivator-1alpha box3 peptide (PGC-1alpha)

Template:ABSTRACT PUBMED 18441008

About this StructureAbout this Structure

3D24 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Communication between the ERR alpha homodimer interface and the PGC-1alpha binding surface vie the helix 8-9 loop., Greschik H, Althage M, Flaig R, Sato Y, Peluso-Iltis C, Chavant V, Choulier L, Cronet P, Rochel N, Schule R, Stromstedt PE, Moras D, J Biol Chem. 2008 Apr 25;. PMID:18441008

Page seeded by OCA on Sun Jul 27 20:29:14 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:3d24.jpg|left|200px]]
+{{Seed}}
+[[Image:3d24.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_3d24|  PDB=3d24  |  SCENE=  }}
-'''Crystal structure of ligand-binding domain of estrogen-related receptor alpha (ERRalpha) in complex with the peroxisome proliferators-activated receptor coactivator-1alpha box3 peptide (PGC-1alpha)'''
+===Crystal structure of ligand-binding domain of estrogen-related receptor alpha (ERRalpha) in complex with the peroxisome proliferators-activated receptor coactivator-1alpha box3 peptide (PGC-1alpha)===
-==Overview==
+<!--
-While structural studies on the ligand-binding domain (LBD) have established the general mode of nuclear receptor (NR)-coactivator interaction, determinants of binding specificity are only partially understood. The LBD of estrogen receptor (ER) alpha, for example, interacts only with a region of peroxisome proliferator-activated receptor coactivator (PGC)-1alpha that contains the canonical LXXLL motif (NR box2), whereas the LBD of estrogen-related receptor (ERR)alpha binds also efficiently an untypical, LXXYL-containing region (NR box3) of PGC-1alpha. Surprisingly, in a previous structural study, the ERalpha LBD has been observed to bind NR box3 of transcriptional intermediary factor (TIF)-2 untypically via LXXYL, while the ERRalpha LBD binds this region of TIF-2 only poorly. Here we present a new crystal structure of the ERRalpha LBD in complex with a PGC-1alpha box3 peptide. In this structure, residues N-terminal of the PGC-1alpha LXXYL motif form contacts with helix 4, the loop connecting helices 8 and 9, and with the C-terminus of the ERRalpha LBD. Interaction studies using wild-type and mutant PGC-1alpha and ERRalpha show that these contacts are functionally relevant and required for efficient ERRalpha/PGC-1alpha interaction. Furthermore, a structure comparison between ERRalpha and ERalpha and mutation analyses provide evidence that the helix 8-9 loop, which differs significantly in both nuclear receptors, is a major determinant of coactivator binding specificity. Finally, our results reveal that in ERRalpha the helix 8-9 loop allosterically links the LBD homodimer interface with the coactivator cleft, thus providing a plausible explanation for distinct PGC-1alpha binding to ERRalpha monomers and homodimers.
+The line below this paragraph, {{ABSTRACT_PUBMED_18441008}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18441008 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18441008}}
 ==About this Structure==
@@ Line 44: / Line 48: @@
 [[Category: Zinc]]
 [[Category: Zinc-finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:50:58 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:29:14 2008''