Rop protein: Difference between revisions
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== Mutants == | == Mutants == | ||
Numerous mutations in the loop region of Rop have been produced: | Numerous mutations in the loop region of Rop have been produced: | ||
*deletion of 5 a/a of loop [[1qx8]] | *deletion of 5 a/a of loop ([[1qx8]]) | ||
*site-directed mutants in loop | *site-directed mutants in loop | ||
*replacement and insertion of glycine residues in loop | *replacement and insertion of glycine residues in loop | ||
*restored of heptad pattern in loop region)[[ | *restored of heptad pattern in loop region) ([[1NKD]]) | ||
*a single alanine to proline substitution [[ | *a single alanine to proline substitution ([[1B6Q]]) (Pro31, unlike Ala31, is more confor-mationally constrained, the dihedral angles of Ala31 in the wild type molecule are prohibited to Pro, and leads to a folding pathway for a thermodynamically less stable conformation.) | ||
*re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle[[ | *re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle([[1YO7]]) | ||
*ALA2ILE2-6, repacted the hydrophobic core and a new fold [[ | *ALA2ILE2-6, repacted the hydrophobic core and a new fold ([[1F4N]]) | ||
{{Clear}} | {{Clear}} | ||