3cs8: Difference between revisions

Revision as of 14:05, 29 July 2008

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File:3cs8.png

Template:STRUCTURE 3cs8

Structural and Biochemical Basis for the Binding Selectivity of PPARg to PGC-1aStructural and Biochemical Basis for the Binding Selectivity of PPARg to PGC-1a

Template:ABSTRACT PUBMED 18469005

About this StructureAbout this Structure

3CS8 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural and biochemical basis for the binding selectivity of PPARgamma to PGC-1alpha., Li Y, Kovach A, Suino-Powell K, Martynowski D, Xu HE, J Biol Chem. 2008 May 9;. PMID:18469005

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-'''Structural and Biochemical Basis for the Binding Selectivity of PPARg to PGC-1a'''
+===Structural and Biochemical Basis for the Binding Selectivity of PPARg to PGC-1a===
-==Overview==
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-The functional interaction between the peroxisome proliferator-activated receptor gamma (PPARgamma) and its coactivator PGC-1alpha is crucial for the normal physiology of PPARgamma and its pharmacological response to antidiabetic treatment with rosiglitazone. Here we report the crystal structure of the PPARgamma ligand binding domain (LBD) bound to rosiglitazone and to a large PGC-1alpha fragment that contains two LXXLL-related motifs. The structure reveals critical contacts mediated through the first LXXLL motif of PGC-1alpha and the PPARgamma coactivator binding site. Through a combination of biochemical and structural studies, we demonstrate that the first LXXLL motif of is the most potent PPARgamma binding motif among all nuclear receptor coactivator motifs tested, and only this motif of the two LXXLL-related motifs in PGC-1alpha is capable of binding to PPARgamma. Our studies reveal that the strong interaction of PGC-1alpha and PPARgamma is mediated through both hydrophobic and specific polar interactions. Mutations within the context of the full-length PGC-1alpha indicate that the first PGC-1alpha motif is necessary and sufficient for PGC-1alpha to coactivate PPARgamma in the presence or absence of rosiglitazone. These results provide a molecular basis for specific recruitment and functional interplay between PPARgamma and PGC-1alpha in glucose homeostasis and adipocyte differentiation.
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 ==About this Structure==
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