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| [[Image:2dqf.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2dqf| PDB=2dqf | SCENE= }} | | {{STRUCTURE_2dqf| PDB=2dqf | SCENE= }} |
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| '''Crystal structure of hyhel-10 FV mutant (y33ay53a) complexed with hen egg lysozyme'''
| | ===Crystal structure of hyhel-10 FV mutant (y33ay53a) complexed with hen egg lysozyme=== |
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| ==Overview==
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| Tyrosine is an important amino acid in protein-protein interaction hot spots. In particular, many Tyr residues are located in the antigen-binding sites of antibodies and endow high affinity and high specificity to these antibodies. To investigate the role of interfacial Tyr residues in protein-protein interactions, we performed crystallographic studies and thermodynamic analyses of the interaction between hen egg lysozyme (HEL) and the anti-HEL antibody HyHEL-10 Fv fragment. HyHEL-10 has six Tyr residues in its antigen-binding site, which were systematically mutated to Phe and Ala using site-directed mutagenesis. The crystal structures revealed several critical roles for these Tyr residues in the interaction between HEL and HyHEL-10 as follows: 1) the aromatic ring of Tyr-50 in the light chain (LTyr-50) was important for the correct ternary structure of variable regions of the immunoglobulin light chain and heavy chain and of HEL; 2) deletion of the hydroxyl group of Tyr-50 in the heavy chain (HTyr-50) resulted in structural changes in the antigen-antibody interface; and 3) the side chains of HTyr-33 and HTyr-53 may help induce fitting of the antibody to the antigen. Hot spot Tyr residues may contribute to the high affinity and high specificity of the antigen-antibody interaction through a diverse set of structural and thermodynamic interactions.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17166830}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17166830 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17166830}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Immune system/hydrolase complex]] | | [[Category: Immune system/hydrolase complex]] |
| [[Category: Mutant]] | | [[Category: Mutant]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:21:19 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:56:18 2008'' |