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| {{STRUCTURE_1uw4| PDB=1uw4 | SCENE= }} | | {{STRUCTURE_1uw4| PDB=1uw4 | SCENE= }} |
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| '''THE STRUCTURAL BASIS OF THE INTERACTION BETWEEN NONSENSE MEDIATED DECAY FACTORS UPF2 AND UPF3'''
| | ===THE STRUCTURAL BASIS OF THE INTERACTION BETWEEN NONSENSE MEDIATED DECAY FACTORS UPF2 AND UPF3=== |
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| ==Overview==
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| Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism by which eukaryotic cells detect and degrade transcripts containing premature termination codons. Three 'up-frameshift' proteins, UPF1, UPF2 and UPF3, are essential for this process in organisms ranging from yeast to human. We present a crystal structure at a resolution of 1.95 A of the complex between the interacting domains of human UPF2 and UPF3b, which are, respectively, a MIF4G (middle portion of eIF4G) domain and an RNP domain (ribonucleoprotein-type RNA-binding domain). The protein-protein interface is mediated by highly conserved charged residues in UPF2 and UPF3b and involves the beta-sheet surface of the UPF3b RNP domain, which is generally used by these domains to bind nucleic acids. We show that the UPF3b RNP does not bind RNA, whereas the UPF2 construct and the complex do. Our results advance understanding of the molecular mechanisms underlying the NMD quality control process.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15004547}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15004547 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15004547}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rna-binding protein]] | | [[Category: Rna-binding protein]] |
| [[Category: Rnp domain]] | | [[Category: Rnp domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:16:50 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:35:48 2008'' |