1b8x: Difference between revisions
New page: left|200px<br /><applet load="1b8x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b8x, resolution 2.70Å" /> '''GLUTATHIONE S-TRANSF... |
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[[Image:1b8x.gif|left|200px]]<br /><applet load="1b8x" size=" | [[Image:1b8x.gif|left|200px]]<br /><applet load="1b8x" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1b8x, resolution 2.70Å" /> | caption="1b8x, resolution 2.70Å" /> | ||
'''GLUTATHIONE S-TRANSFERASE FUSED WITH THE NUCLEAR MATRIX TARGETING SIGNAL OF THE TRANSCRIPTION FACTOR AML-1'''<br /> | '''GLUTATHIONE S-TRANSFERASE FUSED WITH THE NUCLEAR MATRIX TARGETING SIGNAL OF THE TRANSCRIPTION FACTOR AML-1'''<br /> | ||
==Overview== | ==Overview== | ||
A glutathione S-transferase fused with the nuclear matrix targeting signal | A glutathione S-transferase fused with the nuclear matrix targeting signal (GST-NMTS) of AML-1/CBF-alpha2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the precipitant. The NMTS is a 31-amino-acid signal peptide that can target the AML-1/CBF-alpha2 protein to the nuclear matrix. The crystal belongs to tetragonal space group P43212 with unit cell dimensions a = b = 93.4 A, c = 57.6 A. There is one GST-fusion protein per asymmetric unit. Crystals diffracted to at least 2.7 A and are appropriate for structure determination. | ||
==About this Structure== | ==About this Structure== | ||
1B8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | 1B8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8X OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Guo, B.]] | [[Category: Guo, B.]] | ||
[[Category: Lian, J | [[Category: Lian, J B.]] | ||
[[Category: Stein, G | [[Category: Stein, G S.]] | ||
[[Category: Stein, J | [[Category: Stein, J L.]] | ||
[[Category: Tang, L.]] | [[Category: Tang, L.]] | ||
[[Category: Wijnen, A | [[Category: Wijnen, A J.Van.]] | ||
[[Category: Zhou, G | [[Category: Zhou, G W.]] | ||
[[Category: nuclear matrix targeting signal protein]] | [[Category: nuclear matrix targeting signal protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:52:44 2008'' | ||
Revision as of 12:52, 21 February 2008
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GLUTATHIONE S-TRANSFERASE FUSED WITH THE NUCLEAR MATRIX TARGETING SIGNAL OF THE TRANSCRIPTION FACTOR AML-1
OverviewOverview
A glutathione S-transferase fused with the nuclear matrix targeting signal (GST-NMTS) of AML-1/CBF-alpha2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the precipitant. The NMTS is a 31-amino-acid signal peptide that can target the AML-1/CBF-alpha2 protein to the nuclear matrix. The crystal belongs to tetragonal space group P43212 with unit cell dimensions a = b = 93.4 A, c = 57.6 A. There is one GST-fusion protein per asymmetric unit. Crystals diffracted to at least 2.7 A and are appropriate for structure determination.
About this StructureAbout this Structure
1B8X is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML-1/CBF-alpha2., Tang L, Guo B, van Wijnen AJ, Lian JB, Stein JL, Stein GS, Zhou GW, J Struct Biol. 1998 Sep;123(1):83-5. PMID:9774548
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