1qjw: Difference between revisions
New page: left|200px<br /> <applet load="1qjw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qjw, resolution 1.90Å" /> '''CEL6A (Y169F) WITH ... |
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==About this Structure== | ==About this Structure== | ||
1QJW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | 1QJW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]] with NAG, MAN, CD and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]]. Structure known Active Sites: ST1 and ST2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QJW OCA]]. | ||
==Reference== | ==Reference== | ||
Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Ce16A from trichoderma reesei., Zou J, Kleywegt GJ, Stahlberg J, Driguez H, Nerinckx W, Claeyssens M, Koivula A, Teeri TT, Jones TA, Structure. 1999 Sep 15;7(9):1035-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10508787 10508787] | Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Ce16A from trichoderma reesei., Zou J, Kleywegt GJ, Stahlberg J, Driguez H, Nerinckx W, Claeyssens M, Koivula A, Teeri TT, Jones TA, Structure. 1999 Sep 15;7(9):1035-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10508787 10508787] | ||
[[Category: Cellulose 1,4-beta-cellobiosidase]] | |||
[[Category: Hypocrea jecorina]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Jones, T.A.]] | [[Category: Jones, T.A.]] | ||
[[Category: Zou, J.Y.]] | [[Category: Zou, J.Y.]] | ||
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[[Category: hydrolase (o-glycosyl)]] | [[Category: hydrolase (o-glycosyl)]] | ||
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Revision as of 13:27, 30 October 2007
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CEL6A (Y169F) WITH A NON-HYDROLYSABLE CELLOTETRAOSE
OverviewOverview
BACKGROUND: Cel6A is one of the two cellobiohydrolases produced by, Trichoderma reesei. The catalytic core has a structure that is a variation, of the classic TIM barrel. The active site is located inside a tunnel, the, roof of which is formed mainly by a pair of loops. RESULTS: We describe, three new ligand complexes. One is the structure of the wild-type enzyme, in complex with a nonhydrolysable cello-oligosaccharide, methyl, 4-S-beta-cellobiosyl-4-thio-beta-cellobioside (Glc)(2)-S-(Glc)(2), which, differs from a cellotetraose in the nature of the central glycosidic, linkage where a sulphur atom replaces an oxygen atom. The second structure, is a mutant, Y169F, in complex with the same ligand, and the third is the, wild-type enzyme in complex with m-iodobenzyl, ... [(full description)]
About this StructureAbout this Structure
1QJW is a [Single protein] structure of sequence from [Hypocrea jecorina] with NAG, MAN, CD and GOL as [ligands]. Active as [Cellulose 1,4-beta-cellobiosidase], with EC number [3.2.1.91]. Structure known Active Sites: ST1 and ST2. Full crystallographic information is available from [OCA].
ReferenceReference
Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Ce16A from trichoderma reesei., Zou J, Kleywegt GJ, Stahlberg J, Driguez H, Nerinckx W, Claeyssens M, Koivula A, Teeri TT, Jones TA, Structure. 1999 Sep 15;7(9):1035-45. PMID:10508787
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