1b0d: Difference between revisions

Revision as of 12:50, 21 February 2008

Structural effects of monovalent anions on polymorphic lysozyme crystals

OverviewOverview

Understanding direct salt effects on protein crystal polymorphism is addressed by comparing different crystal forms (triclinic, monoclinic, tetragonal and orthorhombic) for hen, turkey, bob white quail and human lysozymes. Four new structures of hen egg-white lysozyme are reported: crystals grown in the presence of NapTS diffracted to 1.85 A, of NaI to 1.6 A, of NaNO(3) to 1.45 A and of KSCN to 1.63 A. These new structures are compared with previously published structures in order to draw a mapping of the surface of different lysozymes interacting with monovalent anions, such as nitrate, chloride, iodide, bromide and thiocyanate. An analysis of the structural sites of these anions in the various lysozyme structures is presented. This study shows common anion sites whatever the crystal form and the chemical nature of anions, while others seem specific to a given geometry and a particular charge environment induced by the crystal packing.

About this StructureAbout this Structure

1B0D is a Single protein structure of sequence from Gallus gallus with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.

ReferenceReference

Structural effects of monovalent anions on polymorphic lysozyme crystals., Vaney MC, Broutin I, Retailleau P, Douangamath A, Lafont S, Hamiaux C, Prange T, Ducruix A, Ries-Kautt M, Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):929-40. Epub 2001, Jun 21. PMID:11418760

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OCA

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-[[Image:1b0d.gif|left|200px]]<br /><applet load="1b0d" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1b0d.gif|left|200px]]<br /><applet load="1b0d" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1b0d, resolution 1.84&Aring;" />
 '''Structural effects of monovalent anions on polymorphic lysozyme crystals'''<br />
 ==Overview==
-Understanding direct salt effects on protein crystal polymorphism is, addressed by comparing different crystal forms (triclinic, monoclinic, tetragonal and orthorhombic) for hen, turkey, bob white quail and human, lysozymes. Four new structures of hen egg-white lysozyme are reported:, crystals grown in the presence of NapTS diffracted to 1.85 A, of NaI to, 1.6 A, of NaNO(3) to 1.45 A and of KSCN to 1.63 A. These new structures, are compared with previously published structures in order to draw a, mapping of the surface of different lysozymes interacting with monovalent, anions, such as nitrate, chloride, iodide, bromide and thiocyanate. An, analysis of the structural sites of these anions in the various lysozyme, structures is presented. This study shows common anion sites whatever the, crystal form and the chemical nature of anions, while others seem specific, to a given geometry and a particular charge environment induced by the, crystal packing.
+Understanding direct salt effects on protein crystal polymorphism is addressed by comparing different crystal forms (triclinic, monoclinic, tetragonal and orthorhombic) for hen, turkey, bob white quail and human lysozymes. Four new structures of hen egg-white lysozyme are reported: crystals grown in the presence of NapTS diffracted to 1.85 A, of NaI to 1.6 A, of NaNO(3) to 1.45 A and of KSCN to 1.63 A. These new structures are compared with previously published structures in order to draw a mapping of the surface of different lysozymes interacting with monovalent anions, such as nitrate, chloride, iodide, bromide and thiocyanate. An analysis of the structural sites of these anions in the various lysozyme structures is presented. This study shows common anion sites whatever the crystal form and the chemical nature of anions, while others seem specific to a given geometry and a particular charge environment induced by the crystal packing.
 ==About this Structure==
-B0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with TSU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B0D OCA].
+B0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=TSU:'>TSU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B0D OCA].
 ==Reference==
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 [[Category: Retailleau, P.]]
 [[Category: Ries-Kautt, M.]]
-[[Category: Vaney, M.C.]]
+[[Category: Vaney, M C.]]
 [[Category: TSU]]
 [[Category: hydrolase (o-glycosyl)]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:15:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:50:10 2008''