1av6: Difference between revisions

Revision as of 12:48, 21 February 2008

VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE

OverviewOverview

Sequence-nonspecific binding of RNA, recognition of a 7-methylguanosine 5' mRNA cap, and methylation of a nucleic acid backbone are three crucial and ubiquitous events in eukaryotic nucleic acid processing and function. These three events occur concurrently in the modification of vaccinia transcripts by the methyltransferase VP39. We report the crystal structure of a ternary complex comprising VP39, coenzyme product S-adenosylhomocysteine, and a 5' m7 G-capped, single-stranded RNA hexamer. This structure reveals a novel and general mechanism for sequence-non-specific recognition of the mRNA transcript in which the protein interacts solely with the sugar-phosphate backbone of a short, single-stranded RNA helix. This report represents the first direct and detailed view of a protein complexed with single-stranded RNA or 5'-capped mRNA.

About this StructureAbout this Structure

1AV6 is a Single protein structure of sequence from Vaccinia virus with and as ligands. Active as Polynucleotide adenylyltransferase, with EC number 2.7.7.19 Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for sequence-nonspecific recognition of 5'-capped mRNA by a cap-modifying enzyme., Hodel AE, Gershon PD, Quiocho FA, Mol Cell. 1998 Feb;1(3):443-7. PMID:9660928

Page seeded by OCA on Thu Feb 21 11:48:35 2008

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OCA

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-[[Image:1av6.gif|left|200px]]<br /><applet load="1av6" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1av6.gif|left|200px]]<br /><applet load="1av6" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1av6, resolution 2.800&Aring;" />
 '''VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE'''<br />
 ==Overview==
-Sequence-nonspecific binding of RNA, recognition of a 7-methylguanosine 5', mRNA cap, and methylation of a nucleic acid backbone are three crucial and, ubiquitous events in eukaryotic nucleic acid processing and function., These three events occur concurrently in the modification of vaccinia, transcripts by the methyltransferase VP39. We report the crystal structure, of a ternary complex comprising VP39, coenzyme product, S-adenosylhomocysteine, and a 5' m7 G-capped, single-stranded RNA hexamer., This structure reveals a novel and general mechanism for, sequence-non-specific recognition of the mRNA transcript in which the, protein interacts solely with the sugar-phosphate backbone of a short, single-stranded RNA helix. This report represents the first direct and, detailed view of a protein complexed with single-stranded RNA or 5'-capped, mRNA.
+Sequence-nonspecific binding of RNA, recognition of a 7-methylguanosine 5' mRNA cap, and methylation of a nucleic acid backbone are three crucial and ubiquitous events in eukaryotic nucleic acid processing and function. These three events occur concurrently in the modification of vaccinia transcripts by the methyltransferase VP39. We report the crystal structure of a ternary complex comprising VP39, coenzyme product S-adenosylhomocysteine, and a 5' m7 G-capped, single-stranded RNA hexamer. This structure reveals a novel and general mechanism for sequence-non-specific recognition of the mRNA transcript in which the protein interacts solely with the sugar-phosphate backbone of a short, single-stranded RNA helix. This report represents the first direct and detailed view of a protein complexed with single-stranded RNA or 5'-capped mRNA.
 ==About this Structure==
-AV6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus] with MGT and SAH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AV6 OCA].
+AV6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus] with <scene name='pdbligand=MGT:'>MGT</scene> and <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AV6 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Vaccinia virus]]
-[[Category: Gershon, P.D.]]
+[[Category: Gershon, P D.]]
-[[Category: Hodel, A.E.]]
+[[Category: Hodel, A E.]]
-[[Category: Quiocho, F.A.]]
+[[Category: Quiocho, F A.]]
 [[Category: MGT]]
 [[Category: SAH]]
@@ Line 28: / Line 28: @@
 [[Category: vaccinia]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:09:05 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:35 2008''