1aoo: Difference between revisions
New page: left|200px<br /><applet load="1aoo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aoo" /> '''AG-SUBSTITUTED METALLOTHIONEIN FROM SACCHARO... |
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[[Image:1aoo.gif|left|200px]]<br /><applet load="1aoo" size=" | [[Image:1aoo.gif|left|200px]]<br /><applet load="1aoo" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''AG-SUBSTITUTED METALLOTHIONEIN FROM SACCHAROMYCES CEREVISIAE, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''AG-SUBSTITUTED METALLOTHIONEIN FROM SACCHAROMYCES CEREVISIAE, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
3D solution structural calculations for yeast silver(I)-substituted | 3D solution structural calculations for yeast silver(I)-substituted metallothionein (MT) and native copper(I) MT were completed using experimentally determined NOE and dihedral angle constraints, in conjunction with experimentally derived metal-to-Cys connectivities for AgMT which were assumed identical for CuMT. For the first 40 residues in both structures, the polypeptide backbone wraps around the metal cluster in two large parallel loops separated by a deep cleft containing the metal cluster. Minor differences between the two structures include differences in hydrogen bonds and the orientation of the N-terminus with the overall protein volume conserved to within 6.5%. | ||
==About this Structure== | ==About this Structure== | ||
1AOO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with AG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1AOO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=AG:'>AG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Armitage, I | [[Category: Armitage, I M.]] | ||
[[Category: Narula, S | [[Category: Narula, S S.]] | ||
[[Category: Peterson, C | [[Category: Peterson, C W.]] | ||
[[Category: AG]] | [[Category: AG]] | ||
[[Category: copper detoxification]] | [[Category: copper detoxification]] | ||
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[[Category: metallothionein]] | [[Category: metallothionein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:46:43 2008'' | ||
Revision as of 12:46, 21 February 2008
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AG-SUBSTITUTED METALLOTHIONEIN FROM SACCHAROMYCES CEREVISIAE, NMR, MINIMIZED AVERAGE STRUCTURE
OverviewOverview
3D solution structural calculations for yeast silver(I)-substituted metallothionein (MT) and native copper(I) MT were completed using experimentally determined NOE and dihedral angle constraints, in conjunction with experimentally derived metal-to-Cys connectivities for AgMT which were assumed identical for CuMT. For the first 40 residues in both structures, the polypeptide backbone wraps around the metal cluster in two large parallel loops separated by a deep cleft containing the metal cluster. Minor differences between the two structures include differences in hydrogen bonds and the orientation of the N-terminus with the overall protein volume conserved to within 6.5%.
About this StructureAbout this Structure
1AOO is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
3D solution structure of copper and silver-substituted yeast metallothioneins., Peterson CW, Narula SS, Armitage IM, FEBS Lett. 1996 Jan 22;379(1):85-93. PMID:8566237
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