2q8b: Difference between revisions
New page: left|200px<br /> <applet load="2q8b" size="450" color="white" frame="true" align="right" spinBox="true" caption="2q8b, resolution 2.30Å" /> '''Structure of the ma... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2q8b. | [[Image:2q8b.jpg|left|200px]]<br /><applet load="2q8b" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2q8b" size=" | |||
caption="2q8b, resolution 2.30Å" /> | caption="2q8b, resolution 2.30Å" /> | ||
'''Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody'''<br /> | '''Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
2Q8B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http:// | 2Q8B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q8B OCA]. | ||
==Reference== | ==Reference== | ||
| Line 22: | Line 21: | ||
[[Category: immune system]] | [[Category: immune system]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:01:06 2008'' | ||
Revision as of 14:01, 23 January 2008
|
Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody
OverviewOverview
Identifying functionally critical regions of the malaria antigen AMA1, (apical membrane antigen 1) is necessary to understand the significance of, the polymorphisms within this antigen for vaccine development. The crystal, structure of AMA1 in complex with the Fab fragment of inhibitory, monoclonal antibody 1F9 reveals that 1F9 binds to the AMA1 solvent-exposed, hydrophobic trough, confirming its importance. 1F9 uses the heavy and, light chain complementarity-determining regions (CDRs) to wrap around the, polymorphic loops adjacent to the trough, but uses a ridge of framework, residues to bind to the hydrophobic trough. The resulting, 1F9-AMA1-combined buried surface of 2,470 A(2) is considerably larger than, previously reported Fab-antigen interfaces. Mutations of polymorphic AMA1, residues within the 1F9 epitope disrupt 1F9 binding and dramatically, reduce the binding of affinity-purified human antibodies. Moreover, 1F9, binding to AMA1 is competed by naturally acquired human antibodies, confirming that the 1F9 epitope is a frequent target of immunological, attack.
About this StructureAbout this Structure
2Q8B is a Single protein structure of sequence from Mus musculus and Plasmodium falciparum. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody., Coley AM, Gupta A, Murphy VJ, Bai T, Kim H, Anders RF, Foley M, Batchelor AH, PLoS Pathog. 2007 Sep 7;3(9):1308-19. PMID:17907804
Page seeded by OCA on Wed Jan 23 13:01:06 2008