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| [[Image:2po4.jpg|left|200px]] | | {{Seed}} |
| | [[Image:2po4.png|left|200px]] |
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| {{STRUCTURE_2po4| PDB=2po4 | SCENE= }} | | {{STRUCTURE_2po4| PDB=2po4 | SCENE= }} |
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| '''X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase'''
| | ===X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase=== |
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| ==Overview==
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| Coliphage N4 virion RNA polymerase (vRNAP), which is injected into the host upon infection, transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure. Here, we describe the 2.0-A resolution x-ray crystal structure of N4 mini-vRNAP, a member of the T7-like, single-unit RNAP family and the minimal component having all RNAP functions of the full-length vRNAP. The structure resembles a "fisted right hand" with Fingers, Palm and Thumb subdomains connected to an N-terminal domain. We established that the specificity loop extending from the Fingers along with W129 of the N-terminal domain play critical roles in hairpin-promoter recognition. A comparison with the structure of the T7 RNAP initiation complex reveals that the pathway of the DNA to the active site is blocked in the apo-form vRNAP, indicating that vRNAP must undergo a large-scale conformational change upon promoter DNA binding and explaining the highly restricted promoter specificity of vRNAP that is essential for phage early transcription.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18362338}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18362338 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18362338}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Right hand shape]] | | [[Category: Right hand shape]] |
| [[Category: Transferase]] | | [[Category: Transferase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:31:16 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:56:26 2008'' |