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| {{STRUCTURE_2pk0| PDB=2pk0 | SCENE= }} | | {{STRUCTURE_2pk0| PDB=2pk0 | SCENE= }} |
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| '''Structure of the S. agalactiae serine/threonine phosphatase at 2.65 resolution'''
| | ===Structure of the S. agalactiae serine/threonine phosphatase at 2.65 resolution=== |
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| ==Overview==
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| We solved the crystal structure of Streptococcus agalactiae serine/threonine phosphatase (SaSTP) using a combination of single-wavelength anomalous dispersion phasing and molecular replacement. The overall structure resembles that of previously characterized members of the PPM/PP2C STP family. The asymmetric unit contains four monomers and we observed two novel conformations for the flap domain among them. In one of these conformations, the enzyme binds three metal ions, whereas in the other it binds only two. The three-metal ion structure also has the active site arginine in a novel conformation. The switch between the two- and three-metal ion structures appears to be binding of another monomer to the active site of STP, which promotes binding of the third metal ion. This interaction may mimic the binding of a product complex, especially since the motif binding to the active site contains a serine residue aligning remarkably well with the phosphate found in the human STP structure.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17521332}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17521332 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17521332}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Streptococcus agalactiae]] | | [[Category: Streptococcus agalactiae]] |
| [[Category: Threonine]] | | [[Category: Threonine]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:16:26 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:44:19 2008'' |