1i3v: Difference between revisions

Revision as of 14:07, 21 February 2008

THREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDED

OverviewOverview

Camelids, camels and llamas, have a unique immune system able to produce heavy-chain only antibodies. Their VH domains (VHHs) are the smallest binding units produced by immune systems, and therefore suitable for biotechnological applications through heterologous expression. The recognition of protein antigens by these VHHs is rather well documented, while less is known about the VHH/hapten interactions. The recently reported X-ray structure of a VHH in complex with a copper-containing azo-dye settled the ability of VHH to recognize haptens by forming a cavity between the three complementarity-determining regions (CDR). Here we report the structures of a VHH (VHH A52) free or complexed with an azo-dye, RR1, without metal ion. The structure of the complex illustrates the involvement of CDR2, CDR3 and a framework residue in a lateral interaction with the hapten. Such a lateral combining site is comparable to that found in classical antibodies, although in the absence of the VL.

About this StructureAbout this Structure

1I3V is a Protein complex structure of sequences from Lama glama. Full crystallographic information is available from OCA.

ReferenceReference

Lateral recognition of a dye hapten by a llama VHH domain., Spinelli S, Tegoni M, Frenken L, van Vliet C, Cambillau C, J Mol Biol. 2001 Aug 3;311(1):123-9. PMID:11469862

Page seeded by OCA on Thu Feb 21 13:07:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1i3v.gif|left|200px]]<br />
+[[Image:1i3v.gif|left|200px]]<br /><applet load="1i3v" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1i3v" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1i3v, resolution 2.03&Aring;" />
 '''THREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDED'''<br />
 ==Overview==
-Camelids, camels and llamas, have a unique immune system able to produce, heavy-chain only antibodies. Their VH domains (VHHs) are the smallest, binding units produced by immune systems, and therefore suitable for, biotechnological applications through heterologous expression. The, recognition of protein antigens by these VHHs is rather well documented, while less is known about the VHH/hapten interactions. The recently, reported X-ray structure of a VHH in complex with a copper-containing, azo-dye settled the ability of VHH to recognize haptens by forming a, cavity between the three complementarity-determining regions (CDR). Here, we report the structures of a VHH (VHH A52) free or complexed with an, azo-dye, RR1, without metal ion. The structure of the complex illustrates, the involvement of CDR2, CDR3 and a framework residue in a lateral, interaction with the hapten. Such a lateral combining site is comparable, to that found in classical antibodies, although in the absence of the VL.
+Camelids, camels and llamas, have a unique immune system able to produce heavy-chain only antibodies. Their VH domains (VHHs) are the smallest binding units produced by immune systems, and therefore suitable for biotechnological applications through heterologous expression. The recognition of protein antigens by these VHHs is rather well documented, while less is known about the VHH/hapten interactions. The recently reported X-ray structure of a VHH in complex with a copper-containing azo-dye settled the ability of VHH to recognize haptens by forming a cavity between the three complementarity-determining regions (CDR). Here we report the structures of a VHH (VHH A52) free or complexed with an azo-dye, RR1, without metal ion. The structure of the complex illustrates the involvement of CDR2, CDR3 and a framework residue in a lateral interaction with the hapten. Such a lateral combining site is comparable to that found in classical antibodies, although in the absence of the VL.
 ==About this Structure==
-I3V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I3V OCA].
+I3V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3V OCA].
 ==Reference==
@@ Line 18: / Line 17: @@
 [[Category: Spinelli, S.]]
 [[Category: Tegoni, M.]]
-[[Category: Vliet, C.van.]]
+[[Category: Vliet, C van.]]
 [[Category: antibody]]
 [[Category: domain vhh]]
 [[Category: lama]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:32:26 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:07:44 2008''