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| {{STRUCTURE_2p8q| PDB=2p8q | SCENE= }} | | {{STRUCTURE_2p8q| PDB=2p8q | SCENE= }} |
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| '''Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain'''
| | ===Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain=== |
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| ==Overview==
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| The nuclear import of uridine-rich ribonucleoproteins (U snRNPs) is mediated by the transport adaptor snurportin 1 (SNP1). Similar to importin alpha, SNP1 uses an N-terminal importin beta binding (sIBB) domain to recruit the receptor importin beta and gain access to the nucleus. In this paper, we demonstrate the sIBB-domain has a bipartite nature, which contains two distinct binding determinants for importin beta. The first determinant spans residues 25-65, and includes the previously identified alphaIBB-region of homology. The second binding determinant encompasses residues 1-24 and resembles region 1011-1035 of the nucleoporin 153 (Nup153). The two binding determinants synergize within the sIBB-domain to confer a low nanomolar binding affinity for importin beta, (Kd ~2nM) in an interaction that, in vitro, is displaced by RanGppNHp. We propose that in vivo the synergy of Nup153 and nuclear RanGTP promotes translocation of U snRNPs into the nucleus. | | The line below this paragraph, {{ABSTRACT_PUBMED_18187419}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18187419 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18187419}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein transport]] | | [[Category: Protein transport]] |
| [[Category: Snurportin]] | | [[Category: Snurportin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:36:59 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:36:36 2008'' |