2oq6: Difference between revisions

Revision as of 18:33, 27 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2oq6.png

Template:STRUCTURE 2oq6

Crystal structure of JMJD2A complexed with histone H3 peptide trimethylated at Lys9Crystal structure of JMJD2A complexed with histone H3 peptide trimethylated at Lys9

Template:ABSTRACT PUBMED 17589501

About this StructureAbout this Structure

2OQ6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of histone demethylase JMJD2A reveal basis for substrate specificity., Ng SS, Kavanagh KL, McDonough MA, Butler D, Pilka ES, Lienard BM, Bray JE, Savitsky P, Gileadi O, von Delft F, Rose NR, Offer J, Scheinost JC, Borowski T, Sundstrom M, Schofield CJ, Oppermann U, Nature. 2007 Jul 5;448(7149):87-91. Epub 2007 Jun 24. PMID:17589501

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OCA

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-'''Crystal structure of JMJD2A complexed with histone H3 peptide trimethylated at Lys9'''
+===Crystal structure of JMJD2A complexed with histone H3 peptide trimethylated at Lys9===
-==Overview==
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-Post-translational histone modification has a fundamental role in chromatin biology and is proposed to constitute a 'histone code' in epigenetic regulation. Differential methylation of histone H3 and H4 lysyl residues regulates processes including heterochromatin formation, X-chromosome inactivation, genome imprinting, DNA repair and transcriptional regulation. The discovery of lysyl demethylases using flavin (amine oxidases) or Fe(II) and 2-oxoglutarate as cofactors (2OG oxygenases) has changed the view of methylation as a stable epigenetic marker. However, little is known about how the demethylases are selective for particular lysyl-containing sequences in specific methylation states, a key to understanding their functions. Here we reveal how human JMJD2A (jumonji domain containing 2A), which is selective towards tri- and dimethylated histone H3 lysyl residues 9 and 36 (H3K9me3/me2 and H3K36me3/me2), discriminates between methylation states and achieves sequence selectivity for H3K9. We report structures of JMJD2A-Ni(II)-Zn(II) inhibitor complexes bound to tri-, di- and monomethyl forms of H3K9 and the trimethyl form of H3K36. The structures reveal a lysyl-binding pocket in which substrates are bound in distinct bent conformations involving the Zn-binding site. We propose a mechanism for achieving methylation state selectivity involving the orientation of the substrate methyl groups towards a ferryl intermediate. The results suggest distinct recognition mechanisms in different demethylase subfamilies and provide a starting point to develop chemical tools for drug discovery and to study and dissect the complexity of reversible histone methylation and its role in chromatin biology.
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 ==About this Structure==
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 [[Category: Structural genomic]]
 [[Category: Structural genomics consortium]]
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