2aai: Difference between revisions
New page: left|200px<br /> <applet load="2aai" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aai, resolution 2.5Å" /> '''CRYSTALLOGRAPHIC REF... |
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'''CRYSTALLOGRAPHIC REFINEMENT OF RICIN TO 2.5 ANGSTROMS'''<br /> | '''CRYSTALLOGRAPHIC REFINEMENT OF RICIN TO 2.5 ANGSTROMS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2AAI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 2AAI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb69_1.html Cholera Toxin]]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http:// | 2AAI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 2AAI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb69_1.html Cholera Toxin]]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: glycosidase]] | [[Category: glycosidase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:14:08 2008'' | ||
Revision as of 18:14, 15 February 2008
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CRYSTALLOGRAPHIC REFINEMENT OF RICIN TO 2.5 ANGSTROMS
OverviewOverview
The plant cytotoxin ricin consists of two disulfide-linked chains, each of, about 30,000 daltons. An initial model based on a 2.8 A MIR electron, density map has been refined against 2.5 A data using rounds of hand, rebuilding coupled with either a restrained least squares algorithm or, molecular dynamics (XPLOR). The last model (9) has an R factor of 21.6%, and RMS deviations from standard bond lengths and angles of 0.021 A and, 4.67 degrees, respectively. Refinement required several peptide segments, in the original model to be adjusted translationally along the electron, density. A wide range of lesser changes were also made. The RMS deviation, of backbone atoms between the original and model 9 was 1.89 A. Molecular, dynamics proved to be a very powerful refinement tool. However, tests, showed that it could not replace human intervention in making adjustments, such as local translations of the peptide chain. The R factor is not a, completely satisfactory indicator of refinement progress; difference, Fouriers, when observed carefully, may be a better monitor.
About this StructureAbout this Structure
2AAI is a Single protein structure of sequence from [1]. The following page contains interesting information on the relation of 2AAI with [Cholera Toxin]. Active as rRNA N-glycosylase, with EC number 3.2.2.22 Full crystallographic information is available from OCA.
ReferenceReference
Crystallographic refinement of ricin to 2.5 A., Rutenber E, Katzin BJ, Ernst S, Collins EJ, Mlsna D, Ready MP, Robertus JD, Proteins. 1991;10(3):240-50. PMID:1881880
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