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| {{STRUCTURE_2o6g| PDB=2o6g | SCENE= }} | | {{STRUCTURE_2o6g| PDB=2o6g | SCENE= }} |
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| '''Crystal structure of IRF-3 bound to the interferon-b enhancer'''
| | ===Crystal structure of IRF-3 bound to the interferon-b enhancer=== |
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| ==Overview==
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| Transcriptional activation of the interferon-beta (IFN-beta) gene requires assembly of an enhanceosome containing ATF-2/c-Jun, IRF-3/IRF-7, and NFkappaB. These factors bind cooperatively to the IFN-beta enhancer and recruit coactivators and chromatin-remodeling proteins to the IFN-beta promoter. We describe here a crystal structure of the DNA-binding domains of IRF-3, IRF-7, and NFkappaB, bound to one half of the enhancer, and use a previously described structure of the remaining half to assemble a complete picture of enhanceosome architecture in the vicinity of the DNA. Association of eight proteins with the enhancer creates a continuous surface for recognizing a composite DNA-binding element. Paucity of local protein-protein contacts suggests that cooperative occupancy of the enhancer comes from both binding-induced changes in DNA conformation and interactions with additional components such as CBP. Contacts with virtually every nucleotide pair account for the evolutionary invariance of the enhancer sequence.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17574024}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17574024 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17574024}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Panne, D.]] | | [[Category: Panne, D.]] |
| [[Category: Protein-dna complex]] | | [[Category: Protein-dna complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:23:12 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:03:32 2008'' |