1fjg: Difference between revisions
New page: left|200px<br /> <applet load="1fjg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fjg, resolution 3.000Å" /> '''STRUCTURE OF THE T... |
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[[Image:1fjg.gif|left|200px]]<br /> | [[Image:1fjg.gif|left|200px]]<br /><applet load="1fjg" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1fjg, resolution 3.000Å" /> | caption="1fjg, resolution 3.000Å" /> | ||
'''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN'''<br /> | '''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN'''<br /> | ||
==Overview== | ==Overview== | ||
The 30S ribosomal subunit has two primary functions in protein synthesis. | The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process. | ||
==About this Structure== | ==About this Structure== | ||
1FJG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with PAR, MG, ZN, SCM and SRY as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1FJG with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb10_1.html Ribosome]]. Full crystallographic information is available from [http:// | 1FJG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=PAR:'>PAR</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SCM:'>SCM</scene> and <scene name='pdbligand=SRY:'>SRY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1FJG with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb10_1.html Ribosome]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJG OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Ribosome]] | [[Category: Ribosome]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Brodersen, D | [[Category: Brodersen, D E.]] | ||
[[Category: Carter, A | [[Category: Carter, A P.]] | ||
[[Category: Jr., W | [[Category: Jr., W M.Clemons.]] | ||
[[Category: Morgan-Warren, R | [[Category: Morgan-Warren, R J.]] | ||
[[Category: Ramakrishnan, V.]] | [[Category: Ramakrishnan, V.]] | ||
[[Category: Wimberly, B | [[Category: Wimberly, B T.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: PAR]] | [[Category: PAR]] | ||
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[[Category: streptomycin]] | [[Category: streptomycin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:16 2008'' | ||
Revision as of 13:39, 21 February 2008
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STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN
OverviewOverview
The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process.
About this StructureAbout this Structure
1FJG is a Protein complex structure of sequences from Thermus thermophilus with , , , and as ligands. The following page contains interesting information on the relation of 1FJG with [Ribosome]. Full crystallographic information is available from OCA.
ReferenceReference
Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics., Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V, Nature. 2000 Sep 21;407(6802):340-8. PMID:11014183
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