2o20: Difference between revisions
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'''Crystal structure of transcription regulator CcpA of Lactococcus lactis''' | '''Crystal structure of transcription regulator CcpA of Lactococcus lactis''' | ||
==Overview== | |||
Catabolite control protein A (CcpA) functions as master transcriptional regulator of carbon catabolism in Firmicutes. It belongs to the family of bacterial repressor/regulator proteins. Here, the crystal structure of the 76 kDa homodimeric CcpA protein from Lactococcus lactis subsp. lactis IL1403 is presented at 1.9 A resolution in the absence of cognate DNA. The phases were derived by molecular replacement and the structure was refined to crystallographic R and R(free) factors of 0.177 and 0.211, respectively. The presence of a sulfate molecule in the direct vicinity of a putative effector-binding site in the monomer allowed the derivation of a model for the possible binding of small organic effector molecules. | |||
==About this Structure== | ==About this Structure== | ||
2O20 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O20 OCA]. | 2O20 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O20 OCA]. | ||
==Reference== | |||
Structure of the transcription regulator CcpA from Lactococcus lactis., Loll B, Kowalczyk M, Alings C, Chieduch A, Bardowski J, Saenger W, Biesiadka J, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):431-6. Epub 2007, Mar 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17372346 17372346] | |||
[[Category: Lactococcus lactis]] | [[Category: Lactococcus lactis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Helix-turn-helix]] | [[Category: Helix-turn-helix]] | ||
[[Category: Transcriptional regulator]] | [[Category: Transcriptional regulator]] | ||
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