2o0f: Difference between revisions

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{{STRUCTURE_2o0f|  PDB=2o0f  |  SCENE=  }}  
{{STRUCTURE_2o0f|  PDB=2o0f  |  SCENE=  }}  


'''Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3'''
===Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3===




==Overview==
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During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important for distinct steps in the action cycle of RF3. Furthermore, we present a cryo-electron microscopy (cryo-EM) structure of the posttermination ribosome bound with RF3 in the GTP form. Our data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center of the ribosome, apparently leading to the release of the class I RF.
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==About this Structure==
==About this Structure==
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[[Category: Rf3]]
[[Category: Rf3]]
[[Category: Ribosome]]
[[Category: Ribosome]]
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