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| [[Image:2o0f.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2o0f| PDB=2o0f | SCENE= }} | | {{STRUCTURE_2o0f| PDB=2o0f | SCENE= }} |
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| '''Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3'''
| | ===Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3=== |
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| ==Overview==
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| During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important for distinct steps in the action cycle of RF3. Furthermore, we present a cryo-electron microscopy (cryo-EM) structure of the posttermination ribosome bound with RF3 in the GTP form. Our data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center of the ribosome, apparently leading to the release of the class I RF.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17540173}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17540173 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17540173}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rf3]] | | [[Category: Rf3]] |
| [[Category: Ribosome]] | | [[Category: Ribosome]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:09:05 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:34:50 2008'' |