1b7t: Difference between revisions
New page: left|200px<br /> <applet load="1b7t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b7t, resolution 2.5Å" /> '''MYOSIN DIGESTED BY P... |
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[[Image:1b7t.gif|left|200px]]<br /> | [[Image:1b7t.gif|left|200px]]<br /><applet load="1b7t" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1b7t" size=" | |||
caption="1b7t, resolution 2.5Å" /> | caption="1b7t, resolution 2.5Å" /> | ||
'''MYOSIN DIGESTED BY PAPAIN'''<br /> | '''MYOSIN DIGESTED BY PAPAIN'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of a proteolytic subfragment from scallop striated | The crystal structure of a proteolytic subfragment from scallop striated muscle myosin, complexed with MgADP, has been solved at 2.5 A resolution and reveals an unusual conformation of the myosin head. The converter and the lever arm are in very different positions from those in either the pre-power stroke or near-rigor state structures; moreover, in contrast to these structures, the SH1 helix is seen to be unwound. Here we compare the overall organization of the myosin head in these three states and show how the conformation of three flexible "joints" produces rearrangements of the four major subdomains in the myosin head with different bound nucleotides. We believe that this novel structure represents one of the prehydrolysis ("ATP") states of the contractile cycle in which the myosin heads stay detached from actin. | ||
==About this Structure== | ==About this Structure== | ||
1B7T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians] with MG, CA and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1B7T with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb18_1.html Myosin]]. Full crystallographic information is available from [http:// | 1B7T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1B7T with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb18_1.html Myosin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7T OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Houdusse, A.]] | [[Category: Houdusse, A.]] | ||
[[Category: Kalabokis, V.]] | [[Category: Kalabokis, V.]] | ||
[[Category: Szent-Gyorgyi, A | [[Category: Szent-Gyorgyi, A G.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: myosin motor]] | [[Category: myosin motor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:52:30 2008'' | ||
Revision as of 12:52, 21 February 2008
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MYOSIN DIGESTED BY PAPAIN
OverviewOverview
The crystal structure of a proteolytic subfragment from scallop striated muscle myosin, complexed with MgADP, has been solved at 2.5 A resolution and reveals an unusual conformation of the myosin head. The converter and the lever arm are in very different positions from those in either the pre-power stroke or near-rigor state structures; moreover, in contrast to these structures, the SH1 helix is seen to be unwound. Here we compare the overall organization of the myosin head in these three states and show how the conformation of three flexible "joints" produces rearrangements of the four major subdomains in the myosin head with different bound nucleotides. We believe that this novel structure represents one of the prehydrolysis ("ATP") states of the contractile cycle in which the myosin heads stay detached from actin.
About this StructureAbout this Structure
1B7T is a Protein complex structure of sequences from Argopecten irradians with , and as ligands. The following page contains interesting information on the relation of 1B7T with [Myosin]. Full crystallographic information is available from OCA.
ReferenceReference
Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head., Houdusse A, Kalabokis VN, Himmel D, Szent-Gyorgyi AG, Cohen C, Cell. 1999 May 14;97(4):459-70. PMID:10338210
Page seeded by OCA on Thu Feb 21 11:52:30 2008