P53: Difference between revisions
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==Exploring the Structure== | ==Exploring the Structure== | ||
Most of the p53 mutations that cause cancer are found in the DNA-binding domain. The most common mutations are shown here, using PDB entry [[1tup]]. This PDB entry includes three copies of the DNA-binding domain; only one (chain B in the file) is shown here. The mutations are found in and around the DNA-binding face of the protein. The most common mutation changes arginine 248, colored red here. Notice how it snakes into the minor groove of the DNA (shown in blue and green), forming a strong stabilizing interaction. When mutated to another amino acid, this interaction is lost. Other key sites of mutation are shown in pink, including arginine residues 175, 249, 273 and 282, and glycine 245. Some of these contact the DNA directly, and others are involved in positioning other DNA-binding amino acids. | Most of the p53 mutations that cause cancer are found in the DNA-binding domain. The most common mutations are shown here, using PDB entry [[1tup]]. This PDB entry includes three copies of the DNA-binding domain; only one (chain B in the file) is shown here. The mutations are found in and around the DNA-binding face of the protein. The most common mutation changes arginine 248, colored red here. Notice how it snakes into the minor groove of the DNA (shown in blue and green), forming a strong stabilizing interaction. When mutated to another amino acid, this interaction is lost. Other key sites of mutation are shown in pink, including arginine residues 175, 249, 273 and 282, and glycine 245. Some of these contact the DNA directly, and others are involved in positioning other DNA-binding amino acids. | ||
<applet load='1tup' size='400' frame='true' align='left' | <applet load='1tup' size='400' frame='true' align='left' script='P53/1tup_mut/1' /> | ||
==Additional information on p53 tumor suppressor== | ==Additional information on p53 tumor suppressor== | ||
*B. Vogelstein, D. Lane, A.J. Levine (2000): Surfing the p53 network. ''Nature'' '''408''', pp. 307-310. | *B. Vogelstein, D. Lane, A.J. Levine (2000): Surfing the p53 network. ''Nature'' '''408''', pp. 307-310. | ||