2bhx: Difference between revisions
New page: left|200px<br /> <applet load="2bhx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bhx, resolution 1.68Å" /> '''RADIATION DAMAGE OF... |
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==About this Structure== | ==About this Structure== | ||
2BHX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_alcalophilus Bacillus alcalophilus]] with MG, CL, PLP, 1PE and PEG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.52 2.6.1.52]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BHX OCA]]. | 2BHX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_alcalophilus Bacillus alcalophilus]] with MG, CL, PLP, 1PE and PEG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Phosphoserine_transaminase Phosphoserine transaminase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.52 2.6.1.52]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BHX OCA]]. | ||
==Reference== | ==Reference== | ||
Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage., Dubnovitsky AP, Ravelli RB, Popov AN, Papageorgiou AC, Protein Sci. 2005 Jun;14(6):1498-507. Epub 2005 May 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15883191 15883191] | Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage., Dubnovitsky AP, Ravelli RB, Popov AN, Papageorgiou AC, Protein Sci. 2005 Jun;14(6):1498-507. Epub 2005 May 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15883191 15883191] | ||
[[Category: Bacillus alcalophilus]] | [[Category: Bacillus alcalophilus]] | ||
[[Category: Phosphoserine transaminase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dubnovitsky, A.P.]] | [[Category: Dubnovitsky, A.P.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
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Revision as of 13:17, 30 October 2007
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RADIATION DAMAGE OF THE SCHIFF BASE IN PHOSPHOSERINE AMINOTRANSFERASE (STRUCTURE A)
OverviewOverview
The X-ray susceptibility of the lysine-pyridoxal-5'-phosphate Schiff base, in Bacillus alcalophilus phosphoserine aminotransferase has been, investigated using crystallographic data collected at 100 K to 1.3 A, resolution, complemented by on-line spectroscopic studies. X-rays induce, deprotonation of the internal aldimine, changes in the Schiff base, conformation, displacement of the cofactor molecule, and disruption of the, Schiff base linkage between pyridoxal-5'-phosphate and the Lys residue., Analysis of the "undamaged" structure reveals a significant chemical, strain on the internal aldimine bond that leads to a pronounced, geometrical distortion of the cofactor. However, upon crystal exposure to, the X-rays, the strain and distortion are relaxed and eventually, diminished when the ... [(full description)]
About this StructureAbout this Structure
2BHX is a [Single protein] structure of sequence from [Bacillus alcalophilus] with MG, CL, PLP, 1PE and PEG as [ligands]. Active as [Phosphoserine transaminase], with EC number [2.6.1.52]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage., Dubnovitsky AP, Ravelli RB, Popov AN, Papageorgiou AC, Protein Sci. 2005 Jun;14(6):1498-507. Epub 2005 May 9. PMID:15883191
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