2jv1: Difference between revisions

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{{STRUCTURE_2jv1|  PDB=2jv1  |  SCENE=  }}  
{{STRUCTURE_2jv1|  PDB=2jv1  |  SCENE=  }}  


'''NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures'''
===NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures===




==Overview==
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Here we present evidence that in water/acetonitrile solvent detailed structural and dynamic information can be obtained for important proteins that are naturally present as oligomers under native conditions. An NMR-derived human insulin monomer structure in H(2)O/CD(3)CN, 65/35 vol%, pH 3.6 is presented and compared with the available X-ray structure of a monomer that forms part of a hexamer (Acta Crystallogr. 2003 Sec. D59, 474) and with NMR structures in water and organic cosolvent. Detailed analysis using PFGSE NMR, temperature-dependent NMR, dilution experiments and CSI proves that the structure is monomeric in the concentration and temperature ranges 0.1-3 mM and 10-30 degrees C, respectively. The presence of long-range interstrand NOEs, as found in the crystal structure of the monomer, provides the evidence for conservation of the tertiary structure. Starting from structures calculated by the program CYANA, two different molecular dynamics simulated annealing refinement protocols were applied, either using the program AMBER in vacuum (AMBER_VC), or including a generalized Born solvent model (AMBER_GB).
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{{ABSTRACT_PUBMED_18040865}}


==Disease==
==Disease==
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==About this Structure==
==About this Structure==
2JV1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JV1 OCA].  
2JV1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JV1 OCA].  


==Reference==
==Reference==
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[[Category: Pharmaceutical]]
[[Category: Pharmaceutical]]
[[Category: Secreted]]
[[Category: Secreted]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:19:13 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:01:33 2008''

Revision as of 17:01, 28 July 2008

File:2jv1.png

Template:STRUCTURE 2jv1

NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structuresNMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures

Template:ABSTRACT PUBMED 18040865

DiseaseDisease

Known disease associated with this structure: Diabetes mellitus, rare form OMIM:[176730], Hyperproinsulinemia, familial OMIM:[176730], MODY, one form OMIM:[176730]

About this StructureAbout this Structure

2JV1 is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

ReferenceReference

Structure of human insulin monomer in water/acetonitrile solution., Bocian W, Sitkowski J, Bednarek E, Tarnowska A, Kawecki R, Kozerski L, J Biomol NMR. 2008 Jan;40(1):55-64. Epub 2007 Nov 27. PMID:18040865

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