|
|
| Line 1: |
Line 1: |
| [[Image:2jpk.jpg|left|200px]] | | {{Seed}} |
| | [[Image:2jpk.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2jpk| PDB=2jpk | SCENE= }} | | {{STRUCTURE_2jpk| PDB=2jpk | SCENE= }} |
|
| |
|
| '''Lactococcin G-b in DPC'''
| | ===Lactococcin G-b in DPC=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| The three-dimensional structures of the two peptides, lactococcin G-alpha (LcnG-alpha; contains 39 residues) and lactococcin G-beta (LcnG-beta, contains 35 residues), that constitute the two-peptide bacteriocin lactococcin G (LcnG) have been determined by nuclear magnetic resonance (NMR) spectroscopy in the presence of DPC micelles and TFE. In DPC, LcnG-alpha has an N-terminal alpha-helix (residues 3-21) that contains a GxxxG helix-helix interaction motif (residues 7-11) and a less well defined C-terminal alpha-helix (residues 24-34), and in between (residues 18-22) there is a second somewhat flexible GxxxG-motif. Its structure in TFE was similar. In DPC, LcnG-beta has an N-terminal alpha-helix (residues 6-19). The region from residues 20 to 35, which also contains a flexible GxxxG-motif (residues 18-22), appeared to be fairly unstructured in DPC. In the presence of TFE, however, the region between and including residues 23 and 32 formed a well defined alpha-helix. The N-terminal helix between and including residues 6 and 19 seen in the presence of DPC, was broken at residues 8 and 9 in the presence of TFE. The N-terminal helices, both in LcnG-alpha and -beta, are amphiphilic. We postulate that LcnG-alpha and -beta have a parallel orientation and interact through helix-helix interactions involving the first GxxxG (residues 7-11) motif in LcnG-alpha and the one (residues 18-22) in LcnG-beta, and that they thus lie in a staggered fashion relative to each other. | | The line below this paragraph, {{ABSTRACT_PUBMED_18187052}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18187052 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_18187052}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| 2JPK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPK OCA]. | | 2JPK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPK OCA]. |
|
| |
|
| ==Reference== | | ==Reference== |
| Line 30: |
Line 34: |
| [[Category: Membrane bound]] | | [[Category: Membrane bound]] |
| [[Category: Peptide]] | | [[Category: Peptide]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:09:21 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:46:51 2008'' |