2jb5: Difference between revisions

Revision as of 14:54, 27 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2jb5.png

Template:STRUCTURE 2jb5

FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1

Template:ABSTRACT PUBMED 18241888

About this StructureAbout this Structure

2JB5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded fab dimer interface., Hillig RC, Urlinger S, Fanghanel J, Brocks B, Haenel C, Stark Y, Sulzle D, Svergun DI, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K, J Mol Biol. 2008 Mar 14;377(1):206-19. Epub 2008 Jan 5. PMID:18241888

Crystallization and molecular-replacement solution of a diagnostic fluorescent dye in complex with a specific Fab fragment., Hillig RC, Baesler S, Urlinger S, Stark Y, Bauer S, Badock V, Huber M, Bahr I, Schirner M, Licha K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):217-23. Epub 2007 Feb 23. PMID:17329818

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 {{STRUCTURE_2jb5|  PDB=2jb5  |  SCENE=  }}
-'''FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1'''
+===FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1===
-==Overview==
+<!--
-Molecular interactions between near-IR fluorescent probes and specific antibodies may be exploited to generate novel smart probes for diagnostic imaging. Using a new phage display technology, we developed such antibody Fab fragments with subnanomolar binding affinity for tetrasulfocyanine, a near-IR in vivo imaging agent. Unexpectedly, some Fabs induced redshifts of the dye absorption peak of up to 44 nm. This is the largest shift reported for a biological system so far. Crystal structure determination and absorption spectroscopy in the crystal in combination with microcalorimetry and small-angle X-ray scattering in solution revealed that the redshift is triggered by formation of a Fab dimer, with tetrasulfocyanine being buried in a fully closed protein cavity within the dimer interface. The derived principle of shifting the absorption peak of a symmetric dye via packaging within a Fab dimer interface may be transferred to other diagnostic fluorophores, opening the way towards smart imaging probes that change their wavelength upon interaction with an antibody.
+The line below this paragraph, {{ABSTRACT_PUBMED_18241888}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18241888 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18241888}}
 ==About this Structure==
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 ==Reference==
 Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded fab dimer interface., Hillig RC, Urlinger S, Fanghanel J, Brocks B, Haenel C, Stark Y, Sulzle D, Svergun DI, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K, J Mol Biol. 2008 Mar 14;377(1):206-19. Epub 2008 Jan 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18241888 18241888]
+Crystallization and molecular-replacement solution of a diagnostic fluorescent dye in complex with a specific Fab fragment., Hillig RC, Baesler S, Urlinger S, Stark Y, Bauer S, Badock V, Huber M, Bahr I, Schirner M, Licha K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):217-23. Epub 2007 Feb 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17329818 17329818]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
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 [[Category: Immunoglobulin domain]]
 [[Category: Tsc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:38:11 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:54:49 2008''