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| {{STRUCTURE_2i4a| PDB=2i4a | SCENE= }} | | {{STRUCTURE_2i4a| PDB=2i4a | SCENE= }} |
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| '''Crystal structure of thioredoxin from the acidophile Acetobacter aceti'''
| | ===Crystal structure of thioredoxin from the acidophile Acetobacter aceti=== |
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| ==Overview==
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| The crystal structure of thioredoxin (AaTrx) from the acetic acid bacterium Acetobacter aceti was determined at 1 A resolution. This is currently the highest resolution crystal structure available for any thioredoxin. Thioredoxins facilitate thiol-disulfide exchange, a process that is expected to be slow at the low pH values encountered in the A. aceti cytoplasm. Despite the apparent need to function at low pH, neither the active site nor the surface charge distribution of AaTrx is notably different from that of Escherichia coli thioredoxin. Apparently the ancestral thioredoxin was sufficiently stable for use in A. aceti or the need to interact with multiple targets constrained the variation of surface residues. The AaTrx structure presented here provides a clear view of all ionizable protein moieties and waters, a first step in understanding how thiol-disulfide exchange might occur in a low pH cytoplasm, and is a basis for biophysical studies of the mechanism of acid-mediated unfolding. The high resolution of this structure should be useful for computational studies of thioredoxin function, protein structure and dynamics, and side-chain ionization. | | The line below this paragraph, {{ABSTRACT_PUBMED_17192591}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17192591 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17192591}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Disulfide exchange]] | | [[Category: Disulfide exchange]] |
| [[Category: Thioredoxin]] | | [[Category: Thioredoxin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:03:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:26:21 2008'' |