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| [[Image:2hv8.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2hv8| PDB=2hv8 | SCENE= }} | | {{STRUCTURE_2hv8| PDB=2hv8 | SCENE= }} |
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| '''Crystal structure of GTP-bound Rab11 in complex with FIP3'''
| | ===Crystal structure of GTP-bound Rab11 in complex with FIP3=== |
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| ==Overview==
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| The Rab11 GTPase regulates recycling of internalized plasma membrane receptors and is essential for completion of cytokinesis. A family of Rab11 interacting proteins (FIPs) that conserve a C-terminal Rab-binding domain (RBD) selectively recognize the active form of Rab11. Normal completion of cytokinesis requires a complex between Rab11 and FIP3. Here, we report the crystal structure and mutational analysis of a heterotetrameric complex between constitutively active Rab11 and a FIP3 construct that includes the RBD. Two Rab11 molecules bind to dyad symmetric sites at the C terminus of FIP3, which forms a non-canonical coiled-coiled dimer with a flared C terminus and hook region. The RBD overlaps with the coiled coil and extends through the C-terminal hook. Although FIP3 engages the switch and interswitch regions of Rab11, the mode of interaction differs significantly from that of other Rab-effector complexes. In particular, the switch II region undergoes a large structural rearrangement from an ordered but non-complementary active conformation to a remodeled conformation that facilitates the interaction with FIP3. Finally, we provide evidence that FIP3 can form homo-oligomers in cells, and that a critical determinant of Rab11 binding in vitro is necessary for FIP3 recruitment to recycling endosomes during cytokinesis. | | The line below this paragraph, {{ABSTRACT_PUBMED_17007872}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17007872 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17007872}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rab11a]] | | [[Category: Rab11a]] |
| [[Category: Recycling endosome]] | | [[Category: Recycling endosome]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:45:08 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:47:01 2008'' |