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| {{STRUCTURE_2huc| PDB=2huc | SCENE= }} | | {{STRUCTURE_2huc| PDB=2huc | SCENE= }} |
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| '''Structural Studies Examining the Substrate Specificity Profiles of PC-PLCBc Protein Variants'''
| | ===Structural Studies Examining the Substrate Specificity Profiles of PC-PLCBc Protein Variants=== |
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| ==Overview==
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| The phosphatidylcholine preferring phospholipase C from Bacillus cereus (PC-PLC(Bc)) catalyzes the hydrolysis of phospholipids in the following order of preference: phosphatidylcholine (PC)>phosphatidylethanolamine (PE)>phosphatidylserine (PS). In previous work, mutagenic, kinetic, and crystallographic experiments suggested that varying the amino acids at the 4th, 56th, and 66th positions had a significant influence upon the substrate specificity profile of PC-PLC(Bc). Here, we report the crystal structures of the native form of several PC-PLC(Bc) variants that exhibited altered substrate specificities for PC, PE, and PS at maximum resolutions of 1.90-2.05 Angstrom. Comparing the structures of these variants to the structure of the wild-type enzyme reveals only minor differences with respect to the number and location of active site water molecules and the side chain conformations of residues at the 4th and 56th positions. These results suggest that subtle changes in steric and electronic properties in the substrate binding site of PC-PLC(Bc) are responsible for the significant changes in substrate selectivity. | | The line below this paragraph, {{ABSTRACT_PUBMED_17324372}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17324372 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17324372}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Plc]] | | [[Category: Plc]] |
| [[Category: Substrate specificity]] | | [[Category: Substrate specificity]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:43:18 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:06:09 2008'' |