2hl4: Difference between revisions

Revision as of 13:49, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2hl4.png

Template:STRUCTURE 2hl4

Crystal structure analysis of human carbonic anhydrase II in complex with a benzenesulfonamide derivativeCrystal structure analysis of human carbonic anhydrase II in complex with a benzenesulfonamide derivative

Template:ABSTRACT PUBMED 17251017

About this StructureAbout this Structure

2HL4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Carbonic anhydrase inhibitors: binding of an antiglaucoma glycosyl-sulfanilamide derivative to human isoform II and its consequences for the drug design of enzyme inhibitors incorporating sugar moieties., Di Fiore A, Scozzafava A, Winum JY, Montero JL, Pedone C, Supuran CT, De Simone G, Bioorg Med Chem Lett. 2007 Mar 15;17(6):1726-31. Epub 2007 Jan 8. PMID:17251017

Refined structure of human carbonic anhydrase II at 2.0 A resolution., Eriksson AE, Jones TA, Liljas A, Proteins. 1988;4(4):274-82. PMID:3151019

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OCA

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-'''Crystal structure analysis of human carbonic anhydrase II in complex with a benzenesulfonamide derivative'''
+===Crystal structure analysis of human carbonic anhydrase II in complex with a benzenesulfonamide derivative===
-==Overview==
+<!--
-N-(4-Sulfamoylphenyl)-alpha-d-glucopyranosylamine, a promising topical antiglaucoma agent, is a potent inhibitor of the zinc enzyme carbonic anhydrase (CA, EC 4.2.1.1). The high resolution X-ray crystal structure of its adduct with the target isoform involved in glaucoma, CA II, is reported here. The sugar sulfanilamide derivative binds to the enzyme in a totally new manner as compared to other CA-inhibitor adducts investigated earlier. The sulfonamide anchor was coordinated to the active site metal ion, and the phenylene ring of the inhibitor filled the channel leading to the active site cavity. The glycosyl moiety responsible for the high water solubility of the compound was oriented towards a hydrophilic region of the active site, where no other inhibitors were observed to be bound up to now. A network of seven hydrogen bonds with four water molecules and the amino acid residues Pro201, Pro202 and Gln92 further stabilize the enzyme-inhibitor adduct. Topiramate, another sugar-based CA inhibitor, binds in a completely different manner to CA II as compared to the sulfonamide investigated here. These findings are useful for the design of potent, sugar-derived enzyme inhibitors.
+The line below this paragraph, {{ABSTRACT_PUBMED_17251017}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17251017 is the PubMed ID number.
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+{{ABSTRACT_PUBMED_17251017}}
 ==About this Structure==
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 ==Reference==
 Carbonic anhydrase inhibitors: binding of an antiglaucoma glycosyl-sulfanilamide derivative to human isoform II and its consequences for the drug design of enzyme inhibitors incorporating sugar moieties., Di Fiore A, Scozzafava A, Winum JY, Montero JL, Pedone C, Supuran CT, De Simone G, Bioorg Med Chem Lett. 2007 Mar 15;17(6):1726-31. Epub 2007 Jan 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17251017 17251017]
+Refined structure of human carbonic anhydrase II at 2.0 A resolution., Eriksson AE, Jones TA, Liljas A, Proteins. 1988;4(4):274-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3151019 3151019]
 [[Category: Carbonate dehydratase]]
 [[Category: Homo sapiens]]
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 [[Category: Winum, J Y.]]
 [[Category: Protein-inhibitor complex]]
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