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| {{STRUCTURE_2h7f| PDB=2h7f | SCENE= }} | | {{STRUCTURE_2h7f| PDB=2h7f | SCENE= }} |
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| '''Structure of variola topoisomerase covalently bound to DNA'''
| | ===Structure of variola topoisomerase covalently bound to DNA=== |
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| ==Overview==
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| Although smallpox has been eradicated from the human population, it is presently feared as a possible agent of bioterrorism. The smallpox virus codes for its own topoisomerase enzyme that differs from its cellular counterpart by requiring a specific DNA sequence for activation of catalysis. Here we present crystal structures of the smallpox virus topoisomerase enzyme bound both covalently and noncovalently to a specific DNA sequence. These structures reveal the basis for site-specific DNA recognition, and they explain how catalysis is likely activated by formation of a specific enzyme-DNA interface. Unexpectedly, the poxvirus enzyme uses a major groove binding alpha helix that is not present in the human enzyme to recognize part of the core recognition sequence and activate the enzyme for catalysis. The topoisomerase-DNA complex structures also provide a three-dimensional framework that may facilitate the rational design of therapeutic agents to treat poxvirus infections.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16885024}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16885024 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16885024}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein-dna complex]] | | [[Category: Protein-dna complex]] |
| [[Category: Type ib topoisomerase]] | | [[Category: Type ib topoisomerase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:57:36 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:55:57 2008'' |