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| {{STRUCTURE_2fts| PDB=2fts | SCENE= }} | | {{STRUCTURE_2fts| PDB=2fts | SCENE= }} |
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| '''Crystal structure of the glycine receptor-gephyrin complex'''
| | ===Crystal structure of the glycine receptor-gephyrin complex=== |
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| ==Overview==
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| Glycine is the major inhibitory neurotransmitter in the spinal cord and brain stem. Gephyrin is required to achieve a high concentration of glycine receptors (GlyRs) in the postsynaptic membrane, which is crucial for efficient glycinergic signal transduction. The interaction between gephyrin and the GlyR involves the E-domain of gephyrin and a cytoplasmic loop located between transmembrane segments three and four of the GlyR beta subunit. Here, we present crystal structures of the gephyrin E-domain with and without the GlyR beta-loop at 2.4 and 2.7 A resolutions, respectively. The GlyR beta-loop is bound in a symmetric 'key and lock' fashion to each E-domain monomer in a pocket adjacent to the dimer interface. Structure-guided mutagenesis followed by in vitro binding and in vivo colocalization assays demonstrate that a hydrophobic interaction formed by Phe 330 of gephyrin and Phe 398 and Ile 400 of the GlyR beta-loop is crucial for binding.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16511563}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16511563 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16511563}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Glycine receptor]] | | [[Category: Glycine receptor]] |
| [[Category: Neuroreceptor anchoring]] | | [[Category: Neuroreceptor anchoring]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:18:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:29:09 2008'' |