2fir: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older editNewer edit →
No edit summary
No edit summary
Line 1: Line 1:
[[Image:2fir.gif|left|200px]]
{{Seed}}
[[Image:2fir.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2fir|  PDB=2fir  |  SCENE=  }}  
{{STRUCTURE_2fir|  PDB=2fir  |  SCENE=  }}  


'''Crystal structure of DFPR-VIIa/sTF'''
===Crystal structure of DFPR-VIIa/sTF===




==Overview==
<!--  
Factor VIIa (FVIIa) consists of a gamma-carboxyglutamic acid (Gla) domain, two epidermal growth factor-like domains, and a protease domain. FVIIa binds seven Ca(2+) ions in the Gla, one in the EGF1, and one in the protease domain. However, blood contains both Ca(2+) and Mg(2+), and the Ca(2+) sites in FVIIa that could be specifically occupied by Mg(2+) are unknown. Furthermore, FVIIa contains a Na(+) and two Zn(2+) sites, but ligands for these cations are undefined. We obtained p-aminobenzamidine-VIIa/soluble tissue factor (sTF) crystals under conditions containing Ca(2+), Mg(2+), Na(+), and Zn(2+). The crystal diffracted to 1.8A resolution, and the final structure has an R-factor of 19.8%. In this structure, the Gla domain has four Ca(2+) and three bound Mg(2+). The EGF1 domain contains one Ca(2+) site, and the protease domain contains one Ca(2+), one Na(+), and two Zn(2+) sites. (45)Ca(2+) binding in the presence/absence of Mg(2+) to FVIIa, Gla-domainless FVIIa, and prothrombin fragment 1 supports the crystal data. Furthermore, unlike in other serine proteases, the amide N of Gly(193) in FVIIa points away from the oxyanion hole in this structure. Importantly, the oxyanion hole is also absent in the benzamidine-FVIIa/sTF structure at 1.87A resolution. However, soaking benzamidine-FVIIa/sTF crystals with d-Phe-Pro-Arg-chloromethyl ketone results in benzamidine displacement, d-Phe-Pro-Arg incorporation, and oxyanion hole formation by a flip of the 192-193 peptide bond in FVIIa. Thus, it is the substrate and not the TF binding that induces oxyanion hole formation and functional active site geometry in FVIIa. Absence of oxyanion hole is unusual and has biologic implications for FVIIa macromolecular substrate specificity and catalysis.
The line below this paragraph, {{ABSTRACT_PUBMED_16757484}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16757484 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_16757484}}


==Disease==
==Disease==
Line 40: Line 44:
[[Category: Soluble tissue factor]]
[[Category: Soluble tissue factor]]
[[Category: Zinc]]
[[Category: Zinc]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:56:45 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:27:34 2008''

Revision as of 13:27, 29 July 2008

File:2fir.png


PDB ID 2fir

Drag the structure with the mouse to rotate
2fir, resolution 2.00Å ()
Ligands: , , , , , , ,
Non-Standard Residues:
Gene: F7 (Homo sapiens), F3 (Homo sapiens)
Activity: Coagulation factor VIIa, with EC number 3.4.21.21
Related: 1dan, 2a2q, 2aer, 2b8o
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Crystal structure of DFPR-VIIa/sTFCrystal structure of DFPR-VIIa/sTF

Template:ABSTRACT PUBMED 16757484

DiseaseDisease

Known disease associated with this structure: Factor VII deficiency OMIM:[227500], Myocardial infarction, decreased susceptibility to OMIM:[227500]

About this StructureAbout this Structure

2FIR is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

High resolution structures of p-aminobenzamidine- and benzamidine-VIIa/soluble tissue factor: unpredicted conformation of the 192-193 peptide bond and mapping of Ca2+, Mg2+, Na+, and Zn2+ sites in factor VIIa., Bajaj SP, Schmidt AE, Agah S, Bajaj MS, Padmanabhan K, J Biol Chem. 2006 Aug 25;281(34):24873-88. Epub 2006 Jun 6. PMID:16757484

Page seeded by OCA on Tue Jul 29 13:27:34 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=2fir&oldid=743158"