2ds0: Difference between revisions

Revision as of 17:32, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2ds0.png

Template:STRUCTURE 2ds0

Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactoseCrystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose

Template:ABSTRACT PUBMED 17234683

About this StructureAbout this Structure

2DS0 is a Single protein structure of sequence from Lumbricus terrestris. Full crystallographic information is available from OCA.

ReferenceReference

Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683

Crystallization and preliminary X-ray crystallographic studies of the C-terminal domain of galactose-binding lectin EW29 from the earthworm Lumbricus terrestris., Suzuki R, Fujimoto Z, Kuno A, Hirabayashi J, Kasai K, Hasegawa T, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1895-6. Epub 2004, Sep 23. PMID:15388944

Novel galactose-binding proteins in Annelida. Characterization of 29-kDa tandem repeat-type lectins from the earthworm Lumbricus terrestris., Hirabayashi J, Dutta SK, Kasai K, J Biol Chem. 1998 Jun 5;273(23):14450-60. PMID:9603958

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 {{STRUCTURE_2ds0|  PDB=2ds0  |  SCENE=  }}
-'''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose'''
+===Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose===
-==Overview==
+<!--
-Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.
+The line below this paragraph, {{ABSTRACT_PUBMED_17234683}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17234683 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17234683}}
 ==About this Structure==
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 ==Reference==
 Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17234683 17234683]
+Crystallization and preliminary X-ray crystallographic studies of the C-terminal domain of galactose-binding lectin EW29 from the earthworm Lumbricus terrestris., Suzuki R, Fujimoto Z, Kuno A, Hirabayashi J, Kasai K, Hasegawa T, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1895-6. Epub 2004, Sep 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15388944 15388944]
+Novel galactose-binding proteins in Annelida. Characterization of 29-kDa tandem repeat-type lectins from the earthworm Lumbricus terrestris., Hirabayashi J, Dutta SK, Kasai K, J Biol Chem. 1998 Jun 5;273(23):14450-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9603958 9603958]
 [[Category: Lumbricus terrestris]]
 [[Category: Single protein]]
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 [[Category: Sugar binding protein]]
 [[Category: Sugar complex]]
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