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| [[Image:2c7m.gif|left|200px]] | | {{Seed}} |
| | [[Image:2c7m.png|left|200px]] |
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| {{STRUCTURE_2c7m| PDB=2c7m | SCENE= }} | | {{STRUCTURE_2c7m| PDB=2c7m | SCENE= }} |
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| '''HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN'''
| | ===HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN=== |
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| ==Overview==
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| The interaction between ubiquitinated proteins and intracellular proteins harboring ubiquitin binding domains (UBDs) is critical to a multitude of cellular processes. Here, we report that Rabex-5, a guanine nucleotide exchange factor for Rab5, binds to Ub through two independent UBDs. These UBDs determine a number of properties of Rabex-5, including its coupled monoubiquitination and interaction in vivo with ubiquitinated EGFRs. Structural and biochemical characterization of the UBDs of Rabex-5 revealed that one of them (MIU, motif interacting with ubiquitin) binds to Ub with modes superimposable to those of the UIM (ubiquitin-interacting motif):Ub interaction, although in the opposite orientation. The other UBD, RUZ (Rabex-5 ubiquitin binding zinc finger) binds to a surface of Ub centered on Asp58(Ub) and distinct from the "canonical" Ile44(Ub)-based surface. The two binding surfaces allow Ub to interact simultaneously with different UBDs, thus opening new perspectives in Ub-mediated signaling. | | The line below this paragraph, {{ABSTRACT_PUBMED_16499958}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16499958 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16499958}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Ubiquitin binding domain]] | | [[Category: Ubiquitin binding domain]] |
| [[Category: Ubl conjugation]] | | [[Category: Ubl conjugation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:23:24 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:26:16 2008'' |