2iv0: Difference between revisions

Revision as of 13:05, 30 October 2007

THERMAL STABILITY OF ISOCITRATE DEHYDROGENASE FROM ARCHAEOGLOBUS FULGIDUS STUDIED BY CRYSTAL STRUCTURE ANALYSIS AND ENGINEERING OF CHIMERS

OverviewOverview

Isocitrate dehydrogenase from Archaeoglobus fulgidus (AfIDH) has an, apparent melting temperature (T (m)) of 98.5 degrees C. To identify the, structural features involved in thermal stabilization of AfIDH, the, structure was solved to 2.5 A resolution. AfIDH was strikingly similar to, mesophilic IDH from Escherichia coli (EcIDH) and displayed almost the same, number of ion pairs and ionic networks. However, two unique inter-domain, networks were present in AfIDH; one three-membered ionic network between, the large and the small domain and one four-membered ionic network between, the clasp and the small domain. The latter ionic network was presumably, reduced in size when the clasp domain of AfIDH was swapped with that of, EcIDH and the T (m) decreased by 18 degrees C. Contrarily, EcIDH ... [(full description)]

About this StructureAbout this Structure

2IV0 is a [Single protein] structure of sequence from [Archaeoglobus fulgidus] with ZN and CL as [ligands]. Active as [Isocitrate dehydrogenase (NADP(+))], with EC number [1.1.1.42]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers., Stokke R, Karlstrom M, Yang N, Leiros I, Ladenstein R, Birkeland NK, Steen IH, Extremophiles. 2007 May;11(3):481-93. Epub 2007 Mar 31. PMID:17401542

Page seeded by OCA on Tue Oct 30 12:10:07 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 ==About this Structure==
-IV0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]] with ZN and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IV0 OCA]].
+IV0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]] with ZN and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IV0 OCA]].
 ==Reference==
 Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers., Stokke R, Karlstrom M, Yang N, Leiros I, Ladenstein R, Birkeland NK, Steen IH, Extremophiles. 2007 May;11(3):481-93. Epub 2007 Mar 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17401542 17401542]
 [[Category: Archaeoglobus fulgidus]]
+[[Category: Isocitrate dehydrogenase (NADP(+))]]
 [[Category: Single protein]]
 [[Category: Birkeland, N.K.]]
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 [[Category: tricarboxylic acid cycle]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:53:51 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:10:07 2007''