2bdb: Difference between revisions

Revision as of 10:11, 23 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2bdb.png

Template:STRUCTURE 2bdb

Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0

Template:ABSTRACT PUBMED 16754679

About this StructureAbout this Structure

2BDB is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

ReferenceReference

Structural analyses on intermediates in serine protease catalysis., Liu B, Schofield CJ, Wilmouth RC, J Biol Chem. 2006 Aug 18;281(33):24024-35. Epub 2006 Jun 5. PMID:16754679

Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:9187653

X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:11473259

X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution., Katona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ, J Biol Chem. 2002 Jun 14;277(24):21962-70. Epub 2002 Mar 14. PMID:11896054

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2bdb.gif|left|200px]]
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 {{STRUCTURE_2bdb|  PDB=2bdb  |  SCENE=  }}
-'''Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0'''
+===Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0===
-==Overview==
+<!--
-Mass spectrometric screening reveals that an unmodified natural heptapeptide--human beta-casomorphin-7, an internal sequence of human beta-casein that possesses opioid-like activity--reacts with porcine pancreatic elastase to form an unusually stable acyl-enzyme complex at low pH. X-ray crystallographic analysis (to 1.9 A resolution) at pH 5 shows continuous electron density linking the C-terminal isoleucine of beta-casomorphin-7 to Ser 195 through an ester bond. The structure reveals a well defined water molecule (Wat 317), equidistant between the carbon of the ester carbonyl and N epsilon 2 of His 57. Deprotonation of Wat 317 will produce a hydroxide ion positioned to attack the ester carbonyl through the favoured Burgi-Dunitz trajectory.
+The line below this paragraph, {{ABSTRACT_PUBMED_16754679}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16754679 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16754679}}
 ==About this Structure==
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 ==Reference==
+Structural analyses on intermediates in serine protease catalysis., Liu B, Schofield CJ, Wilmouth RC, J Biol Chem. 2006 Aug 18;281(33):24024-35. Epub 2006 Jun 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16754679 16754679]
 Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9187653 9187653]
+X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11473259 11473259]
+X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution., Katona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ, J Biol Chem. 2002 Jun 14;277(24):21962-70. Epub 2002 Mar 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11896054 11896054]
 [[Category: Pancreatic elastase]]
 [[Category: Single protein]]
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 [[Category: Schofield, C J.]]
 [[Category: Wilmouth, R C.]]
+[[Category: Hydrolase]]
 [[Category: Serine proteinase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:08:13 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 10:11:46 2008''