2bd7: Difference between revisions

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-[[Image:2bd7.gif|left|200px]]
+{{Seed}}
+[[Image:2bd7.png|left|200px]]
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 {{STRUCTURE_2bd7|  PDB=2bd7  |  SCENE=  }}
-'''Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak)'''
+===Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak)===
-==Overview==
+<!--
-Mass spectrometric screening reveals that an unmodified natural heptapeptide--human beta-casomorphin-7, an internal sequence of human beta-casein that possesses opioid-like activity--reacts with porcine pancreatic elastase to form an unusually stable acyl-enzyme complex at low pH. X-ray crystallographic analysis (to 1.9 A resolution) at pH 5 shows continuous electron density linking the C-terminal isoleucine of beta-casomorphin-7 to Ser 195 through an ester bond. The structure reveals a well defined water molecule (Wat 317), equidistant between the carbon of the ester carbonyl and N epsilon 2 of His 57. Deprotonation of Wat 317 will produce a hydroxide ion positioned to attack the ester carbonyl through the favoured Burgi-Dunitz trajectory.
+The line below this paragraph, {{ABSTRACT_PUBMED_16754679}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16754679 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16754679}}
 ==About this Structure==
-BD7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BD7 OCA].
+BD7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BD7 OCA].
 ==Reference==
+Structural analyses on intermediates in serine protease catalysis., Liu B, Schofield CJ, Wilmouth RC, J Biol Chem. 2006 Aug 18;281(33):24024-35. Epub 2006 Jun 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16754679 16754679]
 Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9187653 9187653]
+X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11473259 11473259]
 [[Category: Pancreatic elastase]]
-[[Category: Single protein]]
+[[Category: Protein complex]]
 [[Category: Sus scrofa]]
 [[Category: Liu, B.]]
 [[Category: Schofield, C J.]]
 [[Category: Wilmouth, R C.]]
+[[Category: Hydrolase]]
 [[Category: Serine proteinase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:08:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 10:09:00 2008''